| Product Number |
OKEH07090 |
| Product Page |
www.avivasysbio.com/nqo1-elisa-kit-rat-okeh07090.html |
| Name |
NQO1 ELISA Kit (Rat) (OKEH07090) |
| NCBI Gene Id |
24314 |
| Gene Full Name |
NAD(P)H quinone dehydrogenase 1 |
| Alias Symbols |
azoreductase;Dia4;Diaphorase (NADH/NADPH);DTD;DT-diaphorase;menadione reductase;NAD(P)H dehydrogenase [quinone] 1;NAD(P)H dehydrogenase, quinone 1;NAD(P)H:menadione oxidoreductase;NAD(P)H:quinone oxidoreductase 1;phylloquinone reductase;QR1;quinone reductase 1. |
| Description of Target |
NAD(P)H dehydrogenase [quinone] 1 |
| Reconstitution and Storage |
Store as indicated in product manual. |
| Datasheets/Manuals |
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS. |
| Specificity |
Natural and recombinant Rat NAD(P)H dehydrogenase [quinone] 1 |
| Assay Info |
Assay Methodology: Quantitative Sandwich ELISA |
| Sensitivity |
0.176 ng/mL |
| Uniprot ID |
https://www.uniprot.org/uniprot/P05982 |
| Protein Name |
P05982 |
| Protein Accession # |
NP_058696 |
| Sample Type |
Serum, Plasma, Tissue Homogenates |
| Nucleotide Accession # |
NM_017000 |
| ELISA Kit Component |
| Component | Amount |
|---|
| Nqo1 Microplate | 96 Wells (12 x 8 Well strips) |
| Nqo1 Lyophilized Standard | 2 x 20 ng |
| 100X Nqo1 HRP-Detector Anitbody | 1 x 120 uL |
| 100X Avidin-HRP Conjugate | 1 x 120 uL |
| Standard Diluent | 1 x 20 mL |
| Detector Antibody Diluent | 1 x 12 mL |
| Conjugate Diluent | 1 x 12 mL |
| 30X Wash Buffer | 1 x 20 mL |
| 100X Luminol Substrate | 1 x 2 mL |
| Substrate Diluent | 1 x 20 mL |
|
| ELISA Kit Reproducibility |
Intra-assay reproducibility was evaluated with 20 replicates of 3 samples representing low, middle and high level LIPC. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level LIPC using 8 replicates on each of 3 plates.
Intra-Assay CV <4.8%
Inter-Assay CV <8.1% |
| ELISA Kit Range |
0.312-20ng/mL |
| ELISA Kit Duration |
~3 Hours |
| ELISA Kit Principle |
Aviva Systems Biology Nqo1 ELISA Kit (Rat) (OKEH07090) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Nqo1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Nqo1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Nqo1 captured in the well. |
| ELISA Kit Recovery |
Mean recovery when spiking into matrix = 100% |
| ELISA Kit Detection Method |
Colorimetric, OD 450 nm |
| Gene Symbol |
Nqo1 |
| Bulk Note |
Please contact customer service when ordering 10+ kits. |
| Predicted Species Reactivity |
Rat, Rattus norvegicus |
| Application |
Enzyme-linked Immunosorbent assay-Sandwich |
| Image 1 | NQO1 ELISA standard curve
 | Rat NQO1 ELISA Kit standard curve |
|
