| Product Number |
OKEH05292 |
| Product Page |
www.avivasysbio.com/aanat-elisa-kit-mouse-okeh05292.html |
| Name |
AANAT ELISA Kit (Mouse) (OKEH05292) |
| Protein Size (# AA) |
205 amino acids |
| NCBI Gene Id |
11298 |
| Gene Full Name |
arylalkylamine N-acetyltransferase |
| Alias Symbols |
AA-NAT;aralkylamine N-acetyltransferase;Na;Nat;Nat-2;Nat4;S;serotonin acetylase;serotonin N-acetyltransferase;Snat. |
| Description of Target |
Serotonin N-acetyltransferase |
| Reconstitution and Storage |
Store as indicated in product manual. |
| Datasheets/Manuals |
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
| Specificity |
Natural and recombinant Mouse Serotonin N-acetyltransferase |
| Assay Info |
Assay Methodology: Quantitative Sandwich ELISA |
| Sensitivity |
0.183 ng/mL |
| Uniprot ID |
https://www.uniprot.org/uniprot/O88816 |
| Protein Name |
O88816 |
| Protein Accession # |
NP_033721.1 |
| Sample Type |
Serum, plasma and other biological fluids |
| Nucleotide Accession # |
NM_009591.3 |
| ELISA Kit Component |
| Component | Amount |
|---|
| AANAT Microplate | 96 Wells (12 x 8 Well strips) |
| AANAT Lyophilized Standard | 2 |
| 100X Biotinylated AANAT Detector Antibody | 1 x 120 uL |
| 100X Avidin-HRP Conjugate | 1 x 120 uL |
| Sample Diluent | 1 x 20 mL |
| Detector Antibody Diluent | 1 x 12 mL |
| Conjugate Diluent | 1 x 12 mL |
| 25X Wash Buffer | 1 x 30 mL |
| Stop Solution | 1 x 10 mL |
| TMB Substrate | 1 x 10 mL |
|
| ELISA Kit Reproducibility |
Three samples concentrations were measured in replicate within an assay plate and across replicate assays to assess Intra- and Mean Inter-Assay Precision.
Mean Intra-Assay Precision - <7.4%, n = 20
Mean Inter-Assay Precision - <9.6%, n = 20 |
| ELISA Kit Range |
0.312-20ng/mL |
| ELISA Kit Duration |
~3 Hours |
| ELISA Kit Principle |
Aviva Systems Biology Aanat ELISA Kit (Mouse) (OKEH05292) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Aanat has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Aanat is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Aanat captured in the well. |
| ELISA Kit Recovery |
Mean recovery when spiking into Serum and Plasma = 86.8% |
| ELISA Kit Detection Method |
Colorimetric, OD450 nm |
| Gene Symbol |
Aanat |
| Bulk Note |
Please contact customer service when ordering 10+ kits. |
| Predicted Species Reactivity |
Mouse, Mus musculus |
| Application |
Enzyme-linked Immunosorbent assay-Sandwich |
| Image 1 | AANAT ELISA Standard Curve
 | Mouse AANAT ELISA Kit Standard Curve |
|
