Abca1 ELISA Kit (Mouse) (OKEH04671)

Data Sheet
 
Product Number OKEH04671
Product Page www.avivasysbio.com/abca1-elisa-kit-mouse-okeh04671.html
Name Abca1 ELISA Kit (Mouse) (OKEH04671)
Protein Size (# AA) 2261 amino acids
Molecular Weight 254 KDa
NCBI Gene Id 11303
Gene Full Name ATP-binding cassette, sub-family A (ABC1), member 1
Alias Symbols ABC;Abc1;ABC-1;ATP-binding cassette 1;ATP-binding cassette sub-family A member 1;ATP-binding cassette transporter 1;phospholipid-transporting ATPase ABCA1.
Description of Target ATP-binding cassette sub-family A member 1
Reconstitution and Storage Store as indicated in product manual.
Datasheets/Manuals Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Specificity Natural and recombinant Mouse ATP-binding cassette sub-family A member 1
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Sensitivity 0.089 ng/mL
Uniprot ID https://www.uniprot.org/uniprot/P41233
Protein Name P41233
Protein Accession # NP_038482.3
Sample Type Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Nucleotide Accession # NM_013454.3
ELISA Kit Component
ComponentAmount
Anti-Abca1 Microplate96 Wells (12 x 8 Well Strips)
Abca1 Lyophilized Standard2
Sample Diluent1 x 20 mL
Abca1 Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
ELISA Kit Reproducibility Mean Intra-Assay CV: < 10%
Mean Inter-Assay CV: < 12%
ELISA Kit Range 0.156-10ng/mL
ELISA Kit Duration ~3 Hours
ELISA Kit Principle Aviva Systems Biology Abca1 ELISA Kit (Mouse) (OKEH04671) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Abca1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Abca1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Abca1 captured in well.
ELISA Kit Detection Method Colorimetric, OD450 nm
Gene Symbol Abca1
Bulk Note Please contact customer service when ordering 10+ kits.
Predicted Species Reactivity Mouse, Mus musculus
Application Enzyme-linked Immunosorbent assay-Sandwich
Image 1
ABCA1 Standard Curve
Mouse Abca1 ELISA Kit standard curve
 

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