| Product Number |
OKEH03955 |
| Product Page |
www.avivasysbio.com/lep-elisa-kit-dog-okeh03955.html |
| Name |
LEP ELISA Kit (Dog) (OKEH03955) |
| Protein Size (# AA) |
167 amino acids |
| Molecular Weight |
167 kDa |
| NCBI Gene Id |
403616 |
| Gene Full Name |
leptin |
| Alias Symbols |
leptin;leptin (obesity homolog, mouse);OB;obesity factor. |
| Description of Target |
Leptin |
| Reconstitution and Storage |
Store as indicated in product manual. |
| Datasheets/Manuals |
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
| Specificity |
Natural and recombinant Dog Leptin |
| Assay Info |
Assay Methodology: Quantitative Sandwich ELISA |
| Sensitivity |
0.092 ng/mL |
| Uniprot ID |
https://www.uniprot.org/uniprot/O02720 |
| Protein Name |
O02720 |
| Protein Accession # |
NP_001003070.1 |
| Sample Type |
Serum, Plasma, Urine, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids |
| Nucleotide Accession # |
NM_001003070.1 |
| ELISA Kit Component |
| Component | Amount |
|---|
| LEP Microplate | 96 Wells (12 x 8 Well strips) |
| LEP Lyophilized Standard | 2 |
| Sample Diluent | 1 x 20 mL |
| 100X Biotinylated LEP Detector Antibody | 1 x 120 uL |
| 100X Avidin-HRP Conjugate | 1 x 120 uL |
| Detector Antibody Diluent | 1 x 10 mL |
| Conjugate Diluent | 1 x 12 mL |
| 25X Wash Buffer | 1 x 30 mL |
| TMB Substrate | 1 x 10 mL |
| Stop Solution | 1 x 10 mL |
|
| ELISA Kit Range |
0.312-20ng/mL |
| ELISA Kit Duration |
~ 3 Hours |
| ELISA Kit Principle |
Aviva Systems Biology LEP ELISA Kit (Dog) (OKEH03955) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LEP has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LEP is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample LEP captured in well. |
| ELISA Kit Detection Method |
Colorimetric, OD450 nm |
| Gene Symbol |
LEP |
| Bulk Note |
Please contact customer service when ordering 10+ kits. |
| Predicted Species Reactivity |
Canis lupus, Dog |
| Application |
Enzyme-linked Immunosorbent assay-Sandwich |
| Image 1 | LEP ELISA standard curve
 | Dog LEP ELISA Kit standard curve |
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