HBA ELISA Kit (Bovine) (OKEH03848)

Data Sheet
 
Product Number OKEH03848
Product Page www.avivasysbio.com/hba-elisa-kit-bovine-okeh03848.html
Name HBA ELISA Kit (Bovine) (OKEH03848)
Protein Size (# AA) 142 amino acids
Molecular Weight 15 kDa
NCBI Gene Id 100140149, 512439
Gene Full Name hemoglobin, alpha 1, hemoglobin, alpha 2
Alias Symbols alpha globin;alpha-globin;HBA2;hemoglobin alpha chain;hemoglobin subunit alpha.
Description of Target Hemoglobin subunit alpha
Reconstitution and Storage Store as indicated in product manual.
Datasheets/Manuals Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
Specificity Natural and recombinant Bovine Hemoglobin subunit alpha
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Sensitivity 0.59 ng/mL
Uniprot ID https://www.uniprot.org/uniprot/P01966
Protein Name P01966
Protein Accession # NP_001070890.2
Sample Type Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Nucleotide Accession # NM_001077422.3
ELISA Kit Component
ComponentAmount
Anti-HBA Microplate96 Wells (12 x 8 Well strips)
HBA Lyophilized Standard2
100X Biotinylated HBA Detector Antibody1 x 120 ÂuL
100X Avidin-HRP Conjugate1 x 120 ÂuL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
ELISA Kit Reproducibility Mean Intra-Assay Precision - ? 4.7%, n = 20
Mean Inter-Assay Precision - ? 8.6%, n = 20
ELISA Kit Range 1.56-100ng/mL
ELISA Kit Duration ~ 3 Hours
ELISA Kit Principle Aviva Systems Biology HBA ELISA Kit (Bovine) (OKEH03848) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HBA has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HBA is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HBA captured in well.
ELISA Kit Recovery Mean recovery when spiking into Serum and Plasma = 87%
ELISA Kit Detection Method Colorimetric, OD450 nm
Gene Symbol HBA, HBA1
Bulk Note Please contact customer service when ordering 10+ kits.
Predicted Species Reactivity Bos taurus, Bovine
Application Enzyme-linked Immunosorbent assay-Sandwich
Image 1
HBA ELISA standard curve
Bovine HBA ELISA Kit standard curve
 

AVIVA SYSTEMS BIOLOGY manufactures and sells quality antibody products covering genome wide proteins.

AVIVA SYSTEMS BIOLOGY
10211 Pacific Mesa Blvd, Ste 401, San Diego, CA 92121 USA | Tel: (858)552-6979 | info@avivasysbio.com