| Product Number |
OKEH03750 |
| Product Page |
www.avivasysbio.com/tnf-elisa-kit-bovine-okeh03750.html |
| Name |
TNF ELISA Kit (Bovine) (OKEH03750) |
| Protein Size (# AA) |
234 amino acids |
| Molecular Weight |
26 kDa |
| NCBI Gene Id |
280943 |
| Gene Full Name |
tumor necrosis factor |
| Alias Symbols |
cachectin;TNFa;TNF-a;TNF-alpha;tumor necrosis factor;tumor necrosis factor alpha;tumor necrosis factor ligand superfamily member 2. |
| Description of Target |
Tumor necrosis factor |
| Reconstitution and Storage |
Store as indicated in product manual. |
| Datasheets/Manuals |
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
| Specificity |
Natural and recombinant Bovine Tumor necrosis factor |
| Assay Info |
Assay Methodology: Quantitative Sandwich ELISA |
| Sensitivity |
0.067 ng/mL |
| Uniprot ID |
https://www.uniprot.org/uniprot/Q06599 |
| Protein Name |
Q06599 |
| Protein Accession # |
NP_776391.2 |
| Sample Type |
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids |
| Nucleotide Accession # |
NM_173966.3 |
| ELISA Kit Component |
| Component | Amount |
|---|
| Anti-TNF Microplate | 96 Wells (12 x 8 Well strips) |
| TNF Lyophilized Standard | 2 |
| Sample Diluent | 1 x 20 mL |
| 100X Biotinylated TNF Detector Antibody | 1 x 120 uL |
| Detector Antibody Diluent | 1 x 12 mL |
| Conjugate Diluent | 1 x 12 mL |
| 25X Wash Buffer | 1 x 30 mL |
| TMB Substrate | 1 x 10 mL |
| Stop Solution | 1 x 10 mL |
|
| ELISA Kit Range |
0.156-10ng/mL |
| ELISA Kit Duration |
~ 3 Hours |
| ELISA Kit Principle |
Aviva Systems Biology TNF ELISA Kit (Bovine) (OKEH03750) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TNF has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TNF is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample TNF captured in well. |
| ELISA Kit Detection Method |
Colorimetric, OD450 nm |
| Gene Symbol |
TNF |
| Bulk Note |
Please contact customer service when ordering 10+ kits. |
| Predicted Species Reactivity |
Bos taurus, Bovine |
| Application |
Enzyme-linked Immunosorbent assay-Sandwich |
| Image 1 | TNF ELISA standard curve
 | Bovine TNF ELISA Kit standard curve |
|
|
