Product Number |
OKEH03748 |
Product Page |
www.avivasysbio.com/il6-elisa-kit-bovine-okeh03748.html |
Name |
IL6 ELISA Kit (Bovine) (OKEH03748) |
Protein Size (# AA) |
205 amino acids |
Molecular Weight |
24 kDa |
NCBI Gene Id |
280826 |
Gene Full Name |
interleukin 6 |
Alias Symbols |
IL-6;interleukin 6 (interferon, beta 2);interleukin-6. |
Description of Target |
Interleukin-6 |
Reconstitution and Storage |
Store as indicated in product manual. |
Datasheets/Manuals |
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. Please click here to view the MSDS. |
Specificity |
Natural and recombinant Bovine Interleukin-6 |
Assay Info |
Assay Methodology: Quantitative Sandwich ELISA |
Sensitivity |
31 pg/mL |
Uniprot ID |
https://www.uniprot.org/uniprot/P26892 |
Protein Name |
P26892 |
Protein Accession # |
NP_776348.1 |
Sample Type |
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids |
Nucleotide Accession # |
NM_173923.2 |
ELISA Kit Component |
Component | Amount |
Anti-IL6 Microplate | 96 Wells (12 x 8 Well strips) |
IL6 Lyophilized Standard | 2 |
100X Avidin-HRP Conjugate | 1 x 120 uL |
Sample Diluent | 1 x 20 mL |
100X Biotinylated IL6 Detector Antibody | 1 x 120 uL |
Detector Antibody Diluent | 1 x 12 mL |
Conjugate Diluent | 1 x 12 mL |
25X Wash Buffer | 1 x 30 mL |
TMB Substrate | 1 x 10 mL |
Stop Solution | 1 x 10 mL |
|
ELISA Kit Range |
78-5000pg/mL |
ELISA Kit Duration |
~ 3 Hours |
ELISA Kit Principle |
Aviva Systems Biology IL6 ELISA Kit (Bovine) (OKEH03748) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IL6 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IL6 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IL6 captured in well. |
ELISA Kit Detection Method |
Colorimetric, OD450 nm |
Gene Symbol |
IL6 |
Bulk Note |
Please contact customer service when ordering 10+ kits. |
Predicted Species Reactivity |
Bos taurus, Bovine |
Application |
Enzyme-linked Immunosorbent assay-Sandwich |
Image 1 | IL6 ELISA standard curve
| Bovine IL6 ELISA Kit standard curve |
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