IL6 ELISA Kit (Bovine) (OKEH03748)

Data Sheet
 
Product Number OKEH03748
Product Page www.avivasysbio.com/il6-elisa-kit-bovine-okeh03748.html
Name IL6 ELISA Kit (Bovine) (OKEH03748)
Protein Size (# AA) 205 amino acids
Molecular Weight 24 kDa
NCBI Gene Id 280826
Gene Full Name interleukin 6
Alias Symbols IL-6;interleukin 6 (interferon, beta 2);interleukin-6.
Description of Target Interleukin-6
Reconstitution and Storage Store as indicated in product manual.
Datasheets/Manuals Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
Specificity Natural and recombinant Bovine Interleukin-6
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Sensitivity 31 pg/mL
Uniprot ID https://www.uniprot.org/uniprot/P26892
Protein Name P26892
Protein Accession # NP_776348.1
Sample Type Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Nucleotide Accession # NM_173923.2
ELISA Kit Component
ComponentAmount
Anti-IL6 Microplate96 Wells (12 x 8 Well strips)
IL6 Lyophilized Standard2
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
100X Biotinylated IL6 Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
ELISA Kit Range 78-5000pg/mL
ELISA Kit Duration ~ 3 Hours
ELISA Kit Principle Aviva Systems Biology IL6 ELISA Kit (Bovine) (OKEH03748) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IL6 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IL6 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IL6 captured in well.
ELISA Kit Detection Method Colorimetric, OD450 nm
Gene Symbol IL6
Bulk Note Please contact customer service when ordering 10+ kits.
Predicted Species Reactivity Bos taurus, Bovine
Application Enzyme-linked Immunosorbent assay-Sandwich
Image 1
IL6 ELISA standard curve
Bovine IL6 ELISA Kit standard curve
 

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