| Product Number |
OKEH03285 |
| Product Page |
www.avivasysbio.com/gstp1-elisa-kit-rat-okeh03285.html |
| Name |
GSTP1 ELISA Kit (Rat) (OKEH03285) |
| Protein Size (# AA) |
210 amino acids |
| Molecular Weight |
23 kDa |
| NCBI Gene Id |
24426 |
| Gene Full Name |
glutathione S-transferase pi 1 |
| Alias Symbols |
chain 7;glutathione S-transferase P;glutathione S-transferase, pi 2;Glutathione-S-transferase, placental enzyme pi type;GST 7-7;GST class-pi;Gst3;Gstp;GST-P;Gstp2. |
| Description of Target |
Glutathione S-transferase P |
| Reconstitution and Storage |
Store as indicated in product manual. |
| Datasheets/Manuals |
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
| Specificity |
Natural and recombinant Rat Glutathione S-transferase P |
| Assay Info |
Assay Methodology: Quantitative Sandwich ELISA |
| Sensitivity |
0.088 ng/mL |
| Uniprot ID |
https://www.uniprot.org/uniprot/P04906 |
| Protein Name |
P04906 |
| Protein Accession # |
NP_036709.1 |
| Sample Type |
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids |
| Nucleotide Accession # |
NM_012577.2 |
| ELISA Kit Component |
| Component | Amount |
|---|
| Anti-Gstp1 Microplate | 96 Wells (12 x 8 Well strips) |
| Gstp1 Lyophilized Standard | 2 |
| Sample Diluent | 1 x 20 mL |
| 100X Biotinylated Gstp1 Detector Antibody | 1 x 120 uL |
| 100X Avidin-HRP Conjugate | 1 x 120 uL |
| Detector Antibody Diluent | 1 x 12 mL |
| Conjugate Diluent | 1 x 12 mL |
| 25X Wash Buffer | 1 x 30 mL |
| TMB Substrate | 1 x 10 mL |
| Stop Solution | 1 x 10 mL |
|
| ELISA Kit Range |
0.156-10ng/mL |
| ELISA Kit Duration |
~ 3 Hours |
| ELISA Kit Principle |
Aviva Systems Biology GSTP1 ELISA Kit (Rat) (OKEH03285) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Gstp1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Gstp1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Gstp1 captured in well. |
| ELISA Kit Detection Method |
Colorimetric, OD450 nm |
| Gene Symbol |
Gstp1 |
| Bulk Note |
Please contact customer service when ordering 10+ kits. |
| Predicted Species Reactivity |
Rat, Rattus norvegicus |
| Application |
Enzyme-linked Immunosorbent assay-Sandwich |
| Image 1 | GSTP1 ELISA standard curve
 | Rat GSTP1 ELISA kit standard curve |
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