| Product Number |
OKEH02977 |
| Product Page |
www.avivasysbio.com/selp-elisa-kit-rat-okeh02977.html |
| Name |
SELP ELISA Kit (Rat) (OKEH02977) |
| Protein Size (# AA) |
768 amino acids |
| Molecular Weight |
84 kDa |
| NCBI Gene Id |
25651 |
| Gene Full Name |
selectin P |
| Alias Symbols |
CD62 antigen-like family member P;GMP-140;granule membrane protein 140;LECAM3;leukocyte-endothelial cell adhesion molecule 3;PADGEM;platelet activation dependent granule-external membrane protein;PSELECT;P-selectin;selectin, platelet. |
| Description of Target |
P-selectin |
| Reconstitution and Storage |
Store as indicated in product manual. |
| Datasheets/Manuals |
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
| Specificity |
Natural and recombinant Rat P-selectin |
| Assay Info |
Assay Methodology: Quantitative Sandwich ELISA |
| Sensitivity |
41.8 pg/mL |
| Uniprot ID |
https://www.uniprot.org/uniprot/P98106 |
| Protein Name |
P98106 |
| Protein Accession # |
NP_037246.1 |
| Sample Type |
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids |
| Nucleotide Accession # |
NM_013114.1 |
| ELISA Kit Component |
| Component | Amount |
|---|
| Anti-Selp Microplate | 96 Wells (12 x 8 Well strips) |
| Selp Lyophilized Standard | 2 |
| 100X Biotinylated Selp Detector Antibody | 1 x 120 uL |
| 100X Avidin-HRP Conjugate | 1 x 120 uL |
| Sample Diluent | 1 x 20 mL |
| Detector Antibody Diluent | 1 x 12 mL |
| Conjugate Diluent | 1 x 12 mL |
| 25X Wash Buffer | 1 x 30 mL |
| TMB Substrate | 1 x 10 mL |
| Stop Solution | 1 x 10 mL |
|
| ELISA Kit Range |
78-5000pg/mL |
| ELISA Kit Duration |
~ 3 Hours |
| ELISA Kit Principle |
Aviva Systems Biology SELP ELISA Kit (Rat) (OKEH02977) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Selp has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Selp is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Selp captured in well. |
| ELISA Kit Detection Method |
Colorimetric, OD450 nm |
| Gene Symbol |
Selp |
| Bulk Note |
Please contact customer service when ordering 10+ kits. |
| Predicted Species Reactivity |
Rat, Rattus norvegicus |
| Application |
Enzyme-linked Immunosorbent assay-Sandwich |
| Image 1 | SELP ELISA standard curve
 | Rat SELP ELISA kit standard curve |
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