Product Number |
OKEH02771 |
Product Page |
www.avivasysbio.com/hif1a-elisa-kit-human-96-wells-okeh02771.html |
Name |
HIF1A ELISA Kit (Human) : 96 Wells (OKEH02771) |
Protein Size (# AA) |
826 amino acids |
Molecular Weight |
93 kDa |
NCBI Gene Id |
3091 |
Gene Full Name |
hypoxia inducible factor 1 subunit alpha |
Alias Symbols |
ARNT interacting protein;ARNT-interacting protein;basic-helix-loop-helix-PAS protein MOP1;bHLHe78;class E basic helix-loop-helix protein 78;HIF1;HIF-1A;HIF-1alpha;HIF-1-alpha;HIF1-ALPHA;hypoxia inducible factor 1 alpha subunit;hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor);hypoxia-inducible factor 1-alpha;hypoxia-inducible factor1alpha;member of PAS protein 1;member of PAS superfamily 1;MOP1;PAS domain-containing protein 8;PAS domain-containing protein 8", "Class E basic helix-loop-helix protein 78", "Member of PAS protein 1;PASD8. |
Description of Target |
Hypoxia-inducible factor 1-alpha |
Reconstitution and Storage |
Store as indicated in product manual. |
Datasheets/Manuals |
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
Specificity |
Natural and recombinant Human Hypoxia-inducible factor 1-alpha |
Assay Info |
Assay Methodology: Quantitative Sandwich ELISA |
Sensitivity |
0.041 ng/mL |
Uniprot ID |
https://www.uniprot.org/uniprot/Q16665 |
Protein Name |
Q16665 |
Protein Accession # |
NP_001230013.1 |
Sample Type |
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids |
Nucleotide Accession # |
NM_001243084.1 |
ELISA Kit Component |
Component | Amount |
Anti-HIF1A Microplate | 96 Wells (12 x 8 Well strips) |
HIF1A Lyophilized Standard | 2 |
100X Biotinylated HIF1A Detector Antibody | 1 x 120 ÂuL |
100X Avidin-HRP Conjugate | 1 x 120 ÂuL |
Sample Diluent | 1 x 20 mL |
Detector Antibody Diluent | 1 x 12 mL |
Conjugate Diluent | 1 x 12 mL |
25X Wash Buffer | 1 x 30 mL |
TMB Substrate | 1 x 10 mL |
Stop Solution | 1 x 10 mL |
|
ELISA Kit Reproducibility |
Mean Intra-assay CV%: < 4.7% (n = 20) Mean Inter-assay CV%: < 6.9% (n = 20) |
ELISA Kit Range |
0.156-10ng/mL |
ELISA Kit Duration |
~ 3 Hours |
ELISA Kit Principle |
Aviva Systems Biology HIF1A ELISA Kit (Human) (OKEH02771) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HIF1A has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HIF1A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HIF1A captured in well. |
ELISA Kit Recovery |
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 100% |
ELISA Kit Detection Method |
Colorimetric, OD450 nm |
COA Datasheet |
Printable COA datasheet for OKEH02771 |
Gene Symbol |
HIF1A |
Bulk Note |
Please contact customer service when ordering 10+ kits. |
Predicted Species Reactivity |
Homo sapiens, Human |
Application |
Enzyme-linked Immunosorbent assay-Sandwich |
Image 1 | HIF1A ELISA
| Human HIF1A ELISA Kit standard curve |
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