HIF1A ELISA Kit (Human) : 96 Wells (OKEH02771)

Data Sheet
 
Product Number OKEH02771
Product Page www.avivasysbio.com/hif1a-elisa-kit-human-96-wells-okeh02771.html
Name HIF1A ELISA Kit (Human) : 96 Wells (OKEH02771)
Protein Size (# AA) 826 amino acids
Molecular Weight 93 kDa
NCBI Gene Id 3091
Gene Full Name hypoxia inducible factor 1 subunit alpha
Alias Symbols ARNT interacting protein;ARNT-interacting protein;basic-helix-loop-helix-PAS protein MOP1;bHLHe78;class E basic helix-loop-helix protein 78;HIF1;HIF-1A;HIF-1alpha;HIF-1-alpha;HIF1-ALPHA;hypoxia inducible factor 1 alpha subunit;hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor);hypoxia-inducible factor 1-alpha;hypoxia-inducible factor1alpha;member of PAS protein 1;member of PAS superfamily 1;MOP1;PAS domain-containing protein 8;PAS domain-containing protein 8", "Class E basic helix-loop-helix protein 78", "Member of PAS protein 1;PASD8.
Description of Target Hypoxia-inducible factor 1-alpha
Reconstitution and Storage Store as indicated in product manual.
Datasheets/Manuals Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Specificity Natural and recombinant Human Hypoxia-inducible factor 1-alpha
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Sensitivity 0.041 ng/mL
Uniprot ID https://www.uniprot.org/uniprot/Q16665
Protein Name Q16665
Protein Accession # NP_001230013.1
Sample Type Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Nucleotide Accession # NM_001243084.1
ELISA Kit Component
ComponentAmount
Anti-HIF1A Microplate96 Wells (12 x 8 Well strips)
HIF1A Lyophilized Standard2
100X Biotinylated HIF1A Detector Antibody1 x 120 ÂuL
100X Avidin-HRP Conjugate1 x 120 ÂuL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
ELISA Kit Reproducibility Mean Intra-assay CV%: < 4.7% (n = 20)
Mean Inter-assay CV%: < 6.9% (n = 20)
ELISA Kit Range 0.156-10ng/mL
ELISA Kit Duration ~ 3 Hours
ELISA Kit Principle Aviva Systems Biology HIF1A ELISA Kit (Human) (OKEH02771) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HIF1A has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HIF1A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HIF1A captured in well.
ELISA Kit Recovery Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 100%
ELISA Kit Detection Method Colorimetric, OD450 nm
COA Datasheet Printable COA datasheet for OKEH02771
Gene Symbol HIF1A
Bulk Note Please contact customer service when ordering 10+ kits.
Predicted Species Reactivity Homo sapiens, Human
Application Enzyme-linked Immunosorbent assay-Sandwich
Image 1
HIF1A ELISA
Human HIF1A ELISA Kit standard curve
 

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