Phosphatidylserine ELISA Kit (OKEH02601)

• Product Number: OKEH02601
• Name: Phosphatidylserine ELISA Kit (OKEH02601)
• Description:
  View product citations for Phosphatidylserine ELISA Kit (OKEH02601) on CiteAb
• Alias Symbols: PS, Phosphatidylserine, PS
• Description of Target: Phosphatidylserine (abbreviated Ptd-L-Ser or PS) is a phospholipid component, usually kept on the inner-leaflet (the cytosolic side) of cell membranes by an enzyme called flippase. When a cell undergoes apoptosis, phosphatidylserine is no longer restricted to the cytosolic part of the membrane, but becomes exposed on the surface of the cell.
• Reconstitution and Storage: Store as indicated in product manual.
• Datasheets/Manuals: Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
• Specificity: Natural and recombinant General Phosphatidylserine
• Additional Information: Target Abbr: PS
  Target Name: Phosphatidylserine
• Additional Information: Pubchem: 6323481
• Additional Information: Chemical formula: C13H24NO10P
• Assay Info: Assay Methodology: Quantitative Competitive ELISA
• Sensitivity: 0.26 ng/mL
• Protein Name: Phosphatidylserine
• Sample Type: Serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids
• ELISA Kit Component:

  Component	Amount
  Anti-Phosphatidylserine Microplate	96 Wells (12 x 8 Well strips)
  Phosphatidylserine Lyophilized Standard	2 x 20 nmol
  Sample Diluent	1 x 20 mL
  100X Phosphatidylserine-Biotin Complex	1 x 60 uL
  100X Avidin-HRP Conjugate	1 x 120 uL
  Biotin Complex Diluent	1 x 12 mL
  Conjugate Diluent	1 x 12 mL
  25X Wash Buffer	1 x 30 mL
  TMB Substrate	1 x 10 mL
  Stop Solution	1 x 10 mL

• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 4.5% (n = 20)
  Mean Inter-assay CV%: < 8.4% (n = 20)
• ELISA Kit Range: 0.78-50ng/mL
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology Phosphatidylserine ELISA Kit (OKEH02601) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Phosphatidylserine antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Phosphatidylserine and incubated. The Phosphatidylserine found in the sample or standards competes with the biotinylated Phosphatidylserine for limited binding sites on the immobilized anti-Phosphatidylserine antibody. Excess unbound biotinylated Phosphatidylserine and sample or standard Phosphatidylserine is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Phosphatidylserine captured in the well and inversely proportional to the amount of Phosphatidylserine which was contained in the sample or standard.
• ELISA Kit Recovery: Mean recovery when spiking into Serum and Plasma = 91%
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• COA Datasheet: Printable COA datasheet for OKEH02601
• Bulk Note: Please contact customer service when ordering 10+ kits.
• Predicted Species Reactivity: All Species
• Application: Enzyme-linked Immunosorbent assay-Competitive
• Image 1: ELISA Standard Curve
  
  Phosphatidylserine ELISA Kit (OKEH02601) standard curve.