| Product Number |
OKEH02452 |
| Product Page |
www.avivasysbio.com/atg5-elisa-kit-human-96-wells-okeh02452.html |
| Name |
ATG5 ELISA Kit (Human) : 96 Wells (OKEH02452) |
| Protein Size (# AA) |
275 amino acids |
| Molecular Weight |
32 kDa |
| NCBI Gene Id |
9474 |
| Gene Full Name |
autophagy related 5 |
| Alias Symbols |
APG5;APG5 autophagy 5-like;APG5L;APG5-LIKE;apoptosis-specific protein;ASP;ATG5 autophagy related 5 homolog;autophagy protein 5;hAPG5;SCAR25. |
| Description of Target |
Autophagy protein 5 |
| Reconstitution and Storage |
Store as indicated in product manual. |
| Datasheets/Manuals |
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
| Specificity |
Natural and recombinant Human Autophagy protein 5 |
| Assay Info |
Assay Methodology: Quantitative Sandwich ELISA |
| Sensitivity |
0.12 ng/mL |
| Uniprot ID |
https://www.uniprot.org/uniprot/Q9H1Y0 |
| Protein Name |
Q9H1Y0 |
| Protein Accession # |
NP_001273035.1 |
| Sample Type |
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids |
| Nucleotide Accession # |
NM_001286106.1 |
| ELISA Kit Component |
| Component | Amount |
|---|
| ATG5 Microplate | 96 Wells (12 x 8 Well strips) |
| ATG5 Lyophilized Standard | 2 |
| Sample Diluent | 1 x 20 mL |
| 100X Biotinylated CYP3A2 Detector Antibody | 1 x 120 uL |
| 100X Avidin-HRP Conjugate | 1 x 120 uL |
| Detector Antibody Diluent | 1 x 12 mL |
| Conjugate Diluent | 1 x 12 mL |
| 25X Wash Buffer | 1 x 30 mL |
| TMB Substrate | 1 x 10 mL |
| Stop Solution | 1 x 10 mL |
|
| ELISA Kit Reproducibility |
Mean Intra-assay CV%: < 6.3% (n = 20)
Mean Inter-assay CV%: < 8.6% (n = 20) |
| ELISA Kit Range |
0.312-20ng/mL |
| ELISA Kit Duration |
~ 3 Hours |
| ELISA Kit Principle |
Aviva Systems Biology ATG5 ELISA Kit (Human) (OKEH02452) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ATG5 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ATG5 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ATG5 captured in well. |
| ELISA Kit Recovery |
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 101% |
| ELISA Kit Detection Method |
Colorimetric, OD450 nm |
| Gene Symbol |
ATG5 |
| Bulk Note |
Please contact customer service when ordering 10+ kits. |
| Predicted Species Reactivity |
Homo sapiens, Human |
| Application |
Enzyme-linked Immunosorbent assay-Sandwich |
| Image 1 | ATG5 ELISA
 | Human ATG5 ELISA Kit standard curve |
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