ABCA7 ELISA Kit (Human) (OKEH02427)

Data Sheet
 
Product Number OKEH02427
Product Page www.avivasysbio.com/abca7-elisa-kit-human-okeh02427.html
Name ABCA7 ELISA Kit (Human) (OKEH02427)
Protein Size (# AA) 2146 amino acids
Molecular Weight 234 kDa
NCBI Gene Id 10347
Gene Full Name ATP binding cassette subfamily A member 7
Alias Symbols ABCA-SSN;ABCX;AD9;ATP-binding cassette sub-family A member 7;ATP-binding cassette, sub-family A (ABC1), member 7;autoantigen SS-N;macrophage ABC transporter;phospholipid-transporting ATPase ABCA7.
Description of Target ATP-binding cassette sub-family A member 7
Reconstitution and Storage Store as indicated in product manual.
Datasheets/Manuals Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Specificity Natural and recombinant Human ATP-binding cassette sub-family A member 7
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Sensitivity 0.045 ng/mL
Uniprot ID https://www.uniprot.org/uniprot/Q8IZY2
Protein Name Q8IZY2
Protein Accession # NP_061985.2
Sample Type Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Nucleotide Accession # NM_019112.3
ELISA Kit Component
ComponentAmount
ABCA7 Microplate96 Wells (12 x 8 Well strips)
ABCA7 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated ABCA7 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
ELISA Kit Reproducibility Mean Intra-assay CV%: < 3.2% (n = 20)
Mean Inter-assay CV%: < 7.4% (n = 20)
ELISA Kit Range 0.156-10ng/ml
ELISA Kit Duration ~ 3 Hours
ELISA Kit Principle Aviva Systems Biology ABCA7 ELISA Kit (Human) (OKEH02427) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ABCA7 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ABCA7 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ABCA7 captured in well.
ELISA Kit Recovery Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 101%
ELISA Kit Detection Method Colorimetric, OD450 nm
Gene Symbol ABCA7
Bulk Note Please contact customer service when ordering 10+ kits.
Predicted Species Reactivity Homo sapiens, Human
Application Enzyme-linked Immunosorbent assay-Sandwich
Image 1
ABCA7 ELISA Standard Curve
Human ABCA7 ELISA Kit standard curve
 

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