PRDX1 ELISA Kit (Human) : 96 Wells (OKEH01572)

Data Sheet
 
Product Number OKEH01572
Product Page www.avivasysbio.com/prdx1-elisa-kit-human-96-wells-okeh01572.html
Name PRDX1 ELISA Kit (Human) : 96 Wells (OKEH01572)
Protein Size (# AA) 199 amino acids
Molecular Weight 22 kDa
NCBI Gene Id 5052
Gene Full Name peroxiredoxin 1
Alias Symbols epididymis secretory sperm binding protein;MSP23;natural killer cell-enhancing factor A;natural killer-enhancing factor A;NKEFA;NKEF-A;PAG;PAGA;PAGB;peroxiredoxin-1;proliferation-associated gene A;proliferation-associated gene protein;PRX1;PRXI;TDPX2;thioredoxin peroxidase 2;thioredoxin-dependent peroxide reductase 2;thioredoxin-dependent peroxiredoxin 1.
Description of Target Peroxiredoxin-1
Reconstitution and Storage Store as indicated in product manual.
Datasheets/Manuals Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Specificity Natural and recombinant Human Peroxiredoxin-1
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Sensitivity 10 pg/mL
Uniprot ID https://www.uniprot.org/uniprot/Q06830
Protein Name Q06830
Protein Accession # NP_001189360.1
Sample Type Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Nucleotide Accession # NM_001202431.1
ELISA Kit Component
ComponentAmount
PRDX1 Microplate96 Wells (12 x 8 Well strips)
PRDX1 Lyophilized Standard2
100X Biotinylated PRDX1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
ELISA Kit Reproducibility Mean Intra-assay CV%: < 4.3% (n = 20)
Mean Inter-assay CV%: < 7.5% (n = 20)
ELISA Kit Range 15.6-1000pg/mL
ELISA Kit Duration ~ 3 Hours
ELISA Kit Principle Aviva Systems Biology PRDX1 ELISA Kit (Human) (OKEH01572) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PRDX1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PRDX1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PRDX1 captured in well.
ELISA Kit Recovery Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 100%
ELISA Kit Detection Method Colorimetric, OD450 nm
Gene Symbol PRDX1
Bulk Note Please contact customer service when ordering 10+ kits.
Predicted Species Reactivity Homo sapiens, Human
Application Enzyme-linked Immunosorbent assay-Sandwich
Image 1
PRDX1 ELISA
Human PRDX1 ELISA Kit standard curve
 

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