Product Number |
OKEH00679 |
Product Page |
www.avivasysbio.com/pinp-elisa-kit-human-okeh00679.html |
Name |
PINP ELISA Kit (Human) (OKEH00679) |
Description |
|
Alias Symbols |
N-terminal propeptide of Collagen alpha-1(I) chain |
Reconstitution and Storage |
Store as indicated in product manual. |
Datasheets/Manuals |
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. Please click here to view the MSDS. |
Specificity |
Natural and recombinant Human N-terminal propeptide of Collagen alpha-1(I) chain |
Assay Info |
Assay Methodology: Quantitative Sandwich ELISA |
Sensitivity |
1.6 ng/mL |
Protein Name |
PINP |
Sample Type |
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids |
ELISA Kit Component |
Component | Amount |
PINP Microplate | 96 Wells (12 x 8 Well strips) |
PINP Lyophilized Standard | 2 |
100X Biotinylated PINP Detector Antibody | 1 x 120 ÂuL |
100X Avidin-HRP Conjugate | 1 x 120 ÂuL |
Sample Diluent | 1 x 20 mL |
Detector Antibody Diluent | 1 x 12 mL |
Conjugate Diluent | 1 x 12 mL |
25X Wash Buffer | 1 x 30 mL |
TMB Substrate | 1 x 10 mL |
Stop Solution | 1 x 10 mL |
|
ELISA Kit Reproducibility |
Mean Intra-assay CV%: < 6.3% (n = 20)
Mean Inter-assay CV%: < 8.5% (n = 20) |
ELISA Kit Range |
3.12-200ng/mL |
ELISA Kit Duration |
~ 3 Hours |
ELISA Kit Principle |
Aviva Systems Biology PINP ELISA Kit (Human) (OKEH00679) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PINP has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PINP is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PINP captured in well. |
ELISA Kit Recovery |
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 107% |
ELISA Kit Detection Method |
Colorimetric, OD 450 nm |
COA Datasheet |
Printable COA datasheet for OKEH00679 |
Gene Symbol |
PINP |
Bulk Note |
Please contact customer service when ordering 10+ kits. |
Predicted Species Reactivity |
Human |
Application |
Enzyme-linked Immunosorbent assay-Sandwich |
Image 1 | PINP ELISA Standard Curve
| Human PINP ELISA Kit standard curve |
|