PINP ELISA Kit (Human) (OKEH00679)

Data Sheet
 
Product Number OKEH00679
Product Page www.avivasysbio.com/pinp-elisa-kit-human-okeh00679.html
Name PINP ELISA Kit (Human) (OKEH00679)
Description
Alias Symbols N-terminal propeptide of Collagen alpha-1(I) chain
Reconstitution and Storage Store as indicated in product manual.
Datasheets/Manuals Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
Specificity Natural and recombinant Human N-terminal propeptide of Collagen alpha-1(I) chain
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Sensitivity 1.6 ng/mL
Protein Name PINP
Sample Type Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
ELISA Kit Component
ComponentAmount
PINP Microplate96 Wells (12 x 8 Well strips)
PINP Lyophilized Standard2
100X Biotinylated PINP Detector Antibody1 x 120 ÂuL
100X Avidin-HRP Conjugate1 x 120 ÂuL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
ELISA Kit Reproducibility Mean Intra-assay CV%: < 6.3% (n = 20)
Mean Inter-assay CV%: < 8.5% (n = 20)
ELISA Kit Range 3.12-200ng/mL
ELISA Kit Duration ~ 3 Hours
ELISA Kit Principle Aviva Systems Biology PINP ELISA Kit (Human) (OKEH00679) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PINP has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PINP is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PINP captured in well.
ELISA Kit Recovery Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 107%
ELISA Kit Detection Method Colorimetric, OD 450 nm
COA Datasheet Printable COA datasheet for OKEH00679
Gene Symbol PINP
Bulk Note Please contact customer service when ordering 10+ kits.
Predicted Species Reactivity Human
Application Enzyme-linked Immunosorbent assay-Sandwich
Image 1
PINP ELISA Standard Curve
Human PINP ELISA Kit standard curve
 

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