| Product Number |
OKEH00593 |
| Product Page |
www.avivasysbio.com/agt-elisa-kit-human-96-wells-okeh00593.html |
| Name |
AGT ELISA Kit (Human) : 96 Wells (OKEH00593) |
| Protein Size (# AA) |
485 amino acids |
| Molecular Weight |
53 kDa |
| NCBI Gene Id |
183 |
| Gene Full Name |
angiotensinogen |
| Alias Symbols |
alpha-1 antiproteinase, antitrypsin;angiotensin I;angiotensin II;angiotensinogen;ANHU;fetal-liver predominant transporter 1;hFLT1;pre-angiotensinogen;serine (or cysteine) proteinase inhibitor;serpin A8;serpin peptidase inhibitor, clade A, member 8;SERPINA8. |
| Description of Target |
Angiotensinogen |
| Reconstitution and Storage |
Store as indicated in product manual. |
| Datasheets/Manuals |
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS. |
| Specificity |
Natural and recombinant Human Angiotensinogen |
| Assay Info |
Assay Methodology: Quantitative Sandwich ELISA |
| Sensitivity |
8.5 pg/mL |
| Uniprot ID |
https://www.uniprot.org/uniprot/P01019 |
| Protein Name |
P01019 |
| Protein Accession # |
NP_000020.1 |
| Sample Type |
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids |
| Nucleotide Accession # |
NM_000029.3 |
| ELISA Kit Component |
| Component | Amount |
|---|
| Anti-AGT Microplate | 96 Wells (12 x 8 Well strips) |
| AGT Lyophilized Standard | 2 |
| Sample Diluent | 1 x 20 mL |
| 100X Biotinylated AGT Detector Antibody | 1 x 120 uL |
| 100X Avidin-HRP Conjugate | 1 x 120 uL |
| Detector Antibody Diluent | 1 x 12 mL |
| Conjugate Diluent | 1 x 12 mL |
| 25X Wash Buffer | 1 x 30 mL |
| TMB Substrate | 1 x 10 mL |
| Stop Solution | 1 x 10 mL |
|
| ELISA Kit Reproducibility |
Mean Intra-assay CV%: < 4.7% (n = 20)
Mean Inter-assay CV%: < 7.5% (n = 20) |
| ELISA Kit Range |
15.6-1000pg/mL |
| ELISA Kit Duration |
~ 3 Hours |
| ELISA Kit Principle |
Aviva Systems Biology AGT ELISA Kit (Human) (OKEH00593) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for AGT has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for AGT is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample AGT captured in well.
|
| ELISA Kit Recovery |
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 85% |
| ELISA Kit Detection Method |
Colorimetric, OD450 nm |
| Gene Symbol |
AGT |
| Bulk Note |
Please contact customer service when ordering 10+ kits. |
| Predicted Species Reactivity |
Homo sapiens, Human |
| Application |
Enzyme-linked Immunosorbent assay-Sandwich |
| Image 1 | AGT ELISA
 | Human AGT ELISA Kit standard curve |
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