ACE2 ELISA Kit (Human) (OKDD00949)

• Product Number: OKDD00949
• Name: ACE2 ELISA Kit (Human) (OKDD00949)
• NCBI Gene Id: 59272
• Gene Full Name: angiotensin converting enzyme 2
• Alias Symbols: ACEH;ACE-related carboxypeptidase;angiotensin I converting enzyme (peptidyl-dipeptidase A) 2;angiotensin I converting enzyme 2;angiotensin-converting enzyme 2;angiotensin-converting enzyme homolog;angiotensin-converting enzyme-related carboxypeptidase;metalloprotease MPROT15;peptidyl-dipeptidase A;truncated angiotensin converting enzyme 2.
• Peptide Sequence: MEPGGARLRLQRTEGLGGERERQPCGDGNTETHRAPDLVQWTRHMEAVKAQLLEQAQGQLRELLDRAMREAIQSYPSQDKPLPPPPPGSLSRTQEPSLGKQKVFIIRKSLLDELMEVQHFRTIYHMFIAGLCVFIISTLAIDFIDEGRLLLEFDLLIFSFGQLPLALVTWVPMFLSTLLAPYQALRLWARGTWTQATGLGCALLAAHAVVLCALPVHVAVEHQLPPASRCVLVFEQVRFLMKSYSFLREAVPGTLRARRGEGIQAPSFSSYLYFLFCPTLIYRETYPRTPYVRWNYVAKNFAQALGCVLYACFILGRLCVPVFANMSREPFSTRALVLSILHATLPGIFMLLLIFFAFLHCWLNAFAEMLRFGDRMFYRDWWNSTSFSNYYRTWNVVVHDWLYSYVYQDGLRLLGARARGVAMLGVFLVSAVAHEYIFCFVLGFFYPVMLILFLVIGGMLNFMMHDQRTGPAWNVLMWTMLFLGQGIQVSLYCQEWYARRHCPLPQATFWGLVTPRSWSCHT
• Description of Target: Essential counter-regulatory carboxypeptidase of the renin-angiotensin hormone system that is a critical regulator of blood volume, systemic vascular resistance, and thus cardiovascular homeostasis (PubMed:27217402). Converts angiotensin I to angiotensin 1-9, a nine-amino acid peptide with anti-hypertrophic effects in cardiomyocytes, and angiotensin II to angiotensin 1-7, which then acts as a beneficial vasodilator and anti-proliferation agent, counterbalancing the actions of the vasoconstrictor angiotensin II (PubMed:10969042, PubMed:10924499, PubMed:11815627, PubMed:19021774, PubMed:14504186). Also removes the C-terminal residue from three other vasoactive peptides, neurotensin, kinetensin, and des-Arg bradykinin, but is not active on bradykinin (PubMed:10969042, PubMed:11815627). Also cleaves other biological peptides, such as apelins (apelin-13, [Pyr1]apelin-13, apelin-17, apelin-36), casomorphins (beta-casomorphin-7, neocasomorphin) and dynorphin A with high efficiency (PubMed:11815627, PubMed:27217402, PubMed:28293165). In addition, ACE2 C-terminus is homologous to collectrin and is responsible for the trafficking of the neutral amino acid transporter SL6A19 to the plasma membrane of gut epithelial cells via direct interaction, regulating its expression on the cell surface and its catalytic activity (PubMed:18424768, PubMed:19185582).
• Reconstitution and Storage: 12 months shelf life. For unopened kits: All the reagents should be kept at 4C upon receipt.
  For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.
• Specificity: This assay has high sensitivity and excellent specificity for detection of ACE2.
  No significant cross-reactivity or interference between ACE2 and analogues was observed.
• Additional Information: Function: Plays a role in lipoprotein assembly and dietary cholesterol absorption. In addition to its acyltransferase activity, it may act as a ligase. May provide cholesteryl esters for lipoprotein secretion from hepatocytes and intestinal mucosa.
• Additional Information: Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions.
  Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA
• Sensitivity: 7.7pg/mL
• Uniprot ID: Q9BYF1
• Protein Name: Angiotensin-converting enzyme 2
• Sample Type: serum, plasma or other biological fluids.
• ELISA Kit Component:

  Component	Amount
  Pre-coated, ready to use 96-well strip plate	1
  Plate sealer for 96 wells	2
  Standard	2
  Standard Diluent	1 x 20mL
  Detection Solution A	1 x 12mL
  Detection Solution B	1 x 12mL
  TMB Substrate	1 x 9mL
  Stop Solution	1 x 6mL
  Wash Buffer (30 x concentrate)	1 x 20mL

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
  Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
  CV(%) = SD/meanX100
  Intra-Assay: CV < 10%
  Inter-Assay: CV < 12%
• ELISA Kit Range: 15.6-1000pg/mL
• ELISA Kit Duration: 1 - 3.5 hours
• ELISA Kit Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +- 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Linearity:

  The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

  Sample	1:2	1:4	1:8	1:16
  serum(n=5)	82-96%	83-98%	81-99%	93-101%
  EDTA plasma(n=5)	88-101%	86-95%	90-102%	80-93%
  heparin plasma(n=5)	80-91%	82-90%	95-104%	79-95%

• ELISA Kit Recovery:

  Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.

  Matrix	Recovery range (%)	Average(%)
  serum(n=5)	81-93	86
  EDTA plasma(n=5)	80-97	88
  heparin plasma(n=5)	90-101	95

• ELISA Kit Detection Method: Colorimetric, OD450 nm
• Gene Symbol: ACE2
• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked immunosorbent assay
• Image 1: ELISA Standard Curve
  
  ELISA Standard Curve