| Product Number |
OKCD04398 |
| Product Page |
www.avivasysbio.com/a1agp-elisa-kit-pig-okcd04398.html |
| Name |
A1AGP ELISA Kit (Pig) (OKCD04398) |
| Description of Target |
Alpha-1 acid glycoprotein |
| Reconstitution and Storage |
2°C to 8°C|-20°C |
| Datasheets/Manuals |
Printable datasheet for A1AGP ELISA Kit (Pig) (OKCD04398) |
| Assay Info |
Assay Methodology: Quantitative Sandwich ELISA |
| Sensitivity |
0.19 ng/mL |
| Uniprot ID |
Q29014 |
| Protein Name |
Alpha-1 acid glycoprotein |
| Sample Type |
Serum, plasma and other biological fluids. |
| ELISA Kit Component |
| Component | Amount |
|---|
| a1agp Microplate | 96 Wells (12 x 8 Well strips) |
| a1agp Lyophilized Standard | 2 |
| 100X a1agp HRP-Detector Anitbody | 1 x 120 uL |
| 100X Avidin-HRP Conjugate | 1 x 120 uL |
| Standard Diluent | 1 x 20 mL |
| Detector Antibody Diluent | 1 x 12 mL |
| Conjugate Diluent | 1 x 12 mL |
| 30X Wash Buffer | 1 x 20 mL |
| TMB Substrate | 1 x 9 mL |
| Stop Solution | 1 x 6 mL |
|
| ELISA Kit Reproducibility |
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Alpha-1-Acid Glycoprotein (a1AGP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Alpha-1-Acid Glycoprotein (a1AGP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% |
| ELISA Kit Range |
0.391-25ng/mL |
| ELISA Kit Duration |
3h |
| ELISA Kit Principle |
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-1-Acid Glycoprotein (a1AGP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alpha-1-Acid Glycoprotein (a1AGP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alpha-1-Acid Glycoprotein (a1AGP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Alpha-1-Acid Glycoprotein (a1AGP) in the samples is then determined by comparing the O.D. of the samples to the standard curve. |
| ELISA Kit Linearity |
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
|---|
| Serum (n=5) | 78-94% | 80-95% | 82-90% | 91-98% |
| EDTA Plasma (n=5) | 99-105% | 78-102% | 88-96% | 79-101% |
| Heparin Plasma (n=5) | 88-101% | 84-104% | 80-95% | 85-96% |
|
| ELISA Kit Recovery |
| Matrix | Recovery range (%) | Average (%) |
|---|
| Serum (n=5) | 96-105 | 101 |
| EDTA Plasma (n=5) | 90-97 | 94 |
| Heparin Plasma (n=5) | 91-105 | 101 |
|
| ELISA Kit Detection Method |
Colorimetric |
| Gene Symbol |
A1AGP |
| Bulk Note |
Please contact customer service when ordering 10+ kits. |
| Predicted Species Reactivity |
Porcine|Sus scrofa |
| Application |
Enzyme-linked Immunosorbent assay-Sandwich |
| Image 1 | |
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