| Product Number |
OKCD04074 |
| Product Page |
www.avivasysbio.com/hmgb1-elisa-kit-human-okcd04074.html |
| Name |
HMGB1 ELISA Kit (Human) (OKCD04074) |
| NCBI Gene Id |
3146 |
| Gene Full Name |
high mobility group box 1 |
| Description |
|
| Alias Symbols |
Amphoterin;High mobility group protein 1;high mobility group protein B1;high-mobility group (nonhistone chromosomal) protein 1;HMG1;HMG-1;HMG3;SBP-1;Sulfoglucuronyl carbohydrate binding protein. |
| Description of Target |
Multifunctional redox sensitive protein with various roles in different cellular compartments. In the nucleus is one of the major chromatin-associated non-histone proteins and acts as a DNA chaperone involved in replication, transcription, chromatin remodeling, V(D)J recombination, DNA repair and genome stability. Proposed to be an universal biosensor for nucleic acids. Promotes host inflammatory response to sterile and infectious signals and is involved in the coordination and integration of innate and adaptive immune responses. In the cytoplasm functions as sensor and/or chaperone for immunogenic nucleic acids implicating the activation of TLR9-mediated immune responses, and mediates autophagy. Acts as danger associated molecular pattern (DAMP) molecule that amplifies immune responses during tissue injury (PubMed:27362237). Released to the extracellular environment can bind DNA, nucleosomes, IL-1 beta, CXCL12, AGER isoform 2/sRAGE, lipopolysaccharide (LPS) and lipoteichoic acid (LTA), and activates cells through engagement of multiple surface receptors. In the extracellular compartment fully reduced HMGB1 (released by necrosis) acts as a chemokine, disulfide HMGB1 (actively secreted) as a cytokine, and sulfonyl HMGB1 (released from apoptotic cells) promotes immunological tolerance (PubMed:23519706, PubMed:23446148, PubMed:23994764, PubMed:25048472). Has proangiogdenic activity (By similarity). May be involved in platelet activation (By similarity). Binds to phosphatidylserine and phosphatidylethanolamide (By similarity). Bound to RAGE mediates signaling for neuronal outgrowth (By similarity). May play a role in accumulation of expanded polyglutamine (polyQ) proteins such as huntingtin (HTT) or TBP (PubMed:23303669, PubMed:25549101). |
| Reconstitution and Storage |
2°C to 8°C|-20°C |
| Datasheets/Manuals |
Printable datasheet for HMGB1 ELISA Kit (Human) (OKCD04074) |
| Protocol Information |
|
| Assay Info |
Assay Methodology: Quantitative Sandwich ELISA |
| Sensitivity |
28.3 pg/mL |
| Uniprot ID |
P09429 |
| Protein Name |
High mobility group protein B1 |
| Publications |
Blood Levels of Free-Circulating Mitochondrial DNA in Septic Shock and Postsurgical Systemic Inflammation and Its Influence on Coagulation: A Secondary Analysis of a Prospective Observational Study. J Clin Med. 9, (2020). 32629885
Dehydrocostus lactone suppresses ox-LDL-induced attachment of monocytes to endothelial cells. Am J Transl Res. 11, 6159-6169 (2019). 31632584
Synthetic lethality between VPS4A and VPS4B triggers an inflammatory response in colorectal cancer. EMBO Mol Med. 12, e10812 (2020). 31930723 |
| Protein Accession # |
NP_065390 |
| Sample Type |
Serum, plasma and other biological fluids |
| Nucleotide Accession # |
NM_000079 |
| ELISA Kit Component |
| Component | Amount |
|---|
| HMGB1 Microplate | 96 Wells (12 x 8 Well strips) |
| HMGB1 Lyophilized Standard | 2 x 3 ng |
| 100X Biotinylated HMGB1 Detector Antibody | 1 x 120 uL |
| 100X Avidin-HRP Conjugate | 1 x 120 uL |
| Standard Diluent | 1 x 20 mL |
| Detector Antibody Diluent | 1 x 12 mL |
| Conjugate Diluent | 1 x 12 mL |
| 30X Wash Buffer | 1 x 20 mL |
| Stop Solution | 1 x 6 mL |
| TMB Substrate | 1 x 9 mL |
|
| ELISA Kit Reproducibility |
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Mobility Group Protein 1 (HMGB1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Mobility Group Protein 1 (HMGB1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% |
| ELISA Kit Range |
62.5-4,000pg/mL |
| ELISA Kit Duration |
3h |
| ELISA Kit Principle |
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Mobility Group Protein 1 (HMGB1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Mobility Group Protein 1 (HMGB1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Mobility Group Protein 1 (HMGB1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of High Mobility Group Protein 1 (HMGB1) in the samples is then determined by comparing the O.D. of the samples to the standard curve. |
| ELISA Kit Linearity |
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|
| Serum (n=5) | 99-105% | 91-101% | 82-97% | 81-104% |
| EDTA Plasma (n=5) | 95-102% | 89-97% | 85-96% | 95-105% |
| Heparin Plasma(n=5) | 91-98% | 80-97% | 80-93% | 98-105% |
|
| ELISA Kit Recovery |
| Matrix | Recovery Range (%) | Average (%) |
|---|
| Serum (n=5) | 96-105 | 101 |
| EDTA Plasma (n=5) | 81-95 | 89 |
| Heparin Plasma(n=5) | 79-90 | 84 |
|
| ELISA Kit Detection Method |
Colorimetric |
| COA Datasheet |
ClickHereto download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
| Gene Symbol |
HMGB1 |
| Bulk Note |
Please contact customer service when ordering 10+ kits. |
| Predicted Species Reactivity |
Homo sapiens|Human |
| Application |
Enzyme-linked Immunosorbent assay-Sandwich |
| Image 1 | ELISA Kit standard curve
 | HMGB1 ELISA Kit (Human) (OKCD04074) Standard Curve |
|
