Product Number |
OKCD02850 |
Product Page |
www.avivasysbio.com/ripk3-elisa-kit-human-okcd02850.html |
Name |
RIPK3 ELISA kit (Human) (OKCD02850) |
Protein Size (# AA) |
518 amino acids |
NCBI Gene Id |
11035 |
Gene Full Name |
receptor interacting serine/threonine kinase 3 |
Alias Symbols |
receptor interacting protein 3;Receptor-interacting protein 3;receptor-interacting serine/threonine-protein kinase 3;RIP3;RIP-3;RIP-like protein kinase 3. |
Description of Target |
Essential for necroptosis, a programmed cell death process in response to death-inducing TNF-alpha family members. Upon induction of necrosis, RIPK3 interacts with, and phosphorylates RIPK1 and MLKL to form a necrosis-inducing complex. RIPK3 binds to and enhances the activity of three metabolic enzymes: GLUL, GLUD1, and PYGL. These metabolic enzymes may eventually stimulate the tricarboxylic acid cycle and oxidative phosphorylation, which could result in enhanced ROS production. |
Reconstitution and Storage |
2°C to 8°C|-20°C |
Datasheets/Manuals |
Printable datasheet for RIPK3 ELISA kit (Human) (OKCD02850) |
Specificity |
This assay has high sensitivity and excellent specificity for detection of Receptor Interacting Serine Threonine Kinase 3 (RIPK3).
No significant cross-reactivity or interference between Receptor Interacting Serine Threonine Kinase 3 (RIPK3) and analogues was observed. |
Protocol Information |
|
Assay Info |
Assay Methodology: Quantitative Sandwich ELISA |
Sensitivity |
0.122 ng/mL |
Uniprot ID |
Q9Y572 |
Protein Name |
Receptor-interacting serine/threonine-protein kinase 3 |
Protein Accession # |
NP_006862.2 |
Sample Type |
Tissue homogenates, cell lysates and other biological fluids |
Nucleotide Accession # |
NM_006871.3 |
ELISA Kit Component |
Component | Amount |
Anti-RIPK3 Microplate | 96 Wells (12 x 8 Well strips) |
RIPK3 Lyophilized Standard | 2 |
100X Biotinylated RIPK3 Detector Antibody | 1 x 120 uL |
100X Avidin-HRP Conjugate | 1 x 120 uL |
Standard Diluent | 1 x 20 mL |
Detector Antibody Diluent | 1 x 12 mL |
Conjugate Diluent | 1 x 12 mL |
30X Wash Buffer | 1 x 20 mL |
Stop Solution | 1 x 6 mL |
TMB Substrate | 1 x 9 mL |
|
ELISA Kit Reproducibility |
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Receptor Interacting Serine Threonine Kinase 3 (RIPK3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Receptor Interacting Serine Threonine Kinase 3 (RIPK3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% |
ELISA Kit Range |
0.312-20ng/mL |
ELISA Kit Duration |
3h |
ELISA Kit Principle |
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Receptor Interacting Serine Threonine Kinase 3 (RIPK3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Receptor Interacting Serine Threonine Kinase 3 (RIPK3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Receptor Interacting Serine Threonine Kinase 3 (RIPK3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Receptor Interacting Serine Threonine Kinase 3 (RIPK3) in the samples is then determined by comparing the O.D. of the samples to the standard curve. |
ELISA Kit Linearity |
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| 89-104% | 97-105% | 88-103% | 83-102% |
|
ELISA Kit Recovery |
Matrix | Recovery range (%) | Average(%) |
| 94-102 | 97 |
|
ELISA Kit Detection Method |
Colorimetric |
Gene Symbol |
RIPK3 |
Bulk Note |
Please contact customer service when ordering 10+ kits. |
Predicted Species Reactivity |
Homo sapiens|Human |
Application |
Enzyme-linked Immunosorbent assay-Sandwich |
Image 1 | RIPK3 ELISA standard curve
| Human RIPK3 ELISA kit standard curve |
|