1-Desamino 8D Arginine Vasopressin ELISA Kit (OKCD02265)

• Product Number: OKCD02265
• Name: 1-Desamino 8D Arginine Vasopressin ELISA Kit (OKCD02265)
• Alias Symbols: DESMOPRESSIN; 16679-58-6; DDAVP; UNII-ENR1LLB0FP
• Description of Target: Desmopressin is a Factor VIII Activator and Vasopressin Analog. The physiologic effect of desmopressin is by means of Increased Coagulation Factor VIII Activity and Increased Coagulation Factor VIII Concentration. The chemical classification of desmopressin is Vasopressins.
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Additional Information: CAS: 16679-58-6
  PubChem: 5311065
• Assay Info: Assay Methodology: Competitive Inhibition Immunoassay
• Sensitivity: 39.5 pg/mL
• Protein Name: 1-Desamino 8D Arginine Vasopressin
• Sample Type: Serum, plasma and other biological fluids.
• ELISA Kit Component:

  Component	Amount
  Anti-1-Desamino 8D Arginine Vasopressin Microplate	96 Wells (12 x 8 Well Strips)
  1-Desamino 8D Arginine Vasopressin Lyophilized Standard	2
  100X 1-Desamino 8D Arginine Vasopressin Biotin Complex	120 uL
  100X Avidin/HRP Conjugate	120 uL
  Standard Diluent	1 x 20 mL
  Biotin Complex Diluent	1 x 12 mL
  Avidin/HRP Conjugate Diluent	1 x 12 mL
  30X Wash Buffer	1 x 20 mL
  TMB Substrate	1 x 9 mL
  Stop Solution	1 x 6 mL

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level 1-Desamino 8D Arginine Vasopressin (DDAVP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level 1-Desamino 8D Arginine Vasopressin (DDAVP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Range: 246.9-20,000pg/mL
• ELISA Kit Duration: 2h
• ELISA Kit Principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to 1-Desamino 8D Arginine Vasopressin (DDAVP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled 1-Desamino 8D Arginine Vasopressin (DDAVP) and unlabeled 1-Desamino 8D Arginine Vasopressin (DDAVP) (Standards or samples) with the pre-coated antibody specific to 1-Desamino 8D Arginine Vasopressin (DDAVP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of 1-Desamino 8D Arginine Vasopressin (DDAVP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of 1-Desamino 8D Arginine Vasopressin (DDAVP) in the sample.
• ELISA Kit Linearity:

  Matrix	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	83-94%	99-105%	96-103%	83-101%
  EDTA plasma(n=5)	83-90%	82-89%	78-99%	96-103%
  heparin plasma(n=5)	88-95%	80-91%	78-102%	78-89%

• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	84-105	102
  EDTA plasma(n=5)	90-101	93
  heparin plasma(n=5)	78-96	83

• ELISA Kit Detection Method: Colorimetric
• Gene Symbol: DDAVP
• Bulk Note: Please contact customer service when ordering 10+ kits.
• Predicted Species Reactivity: All Species
• Application: Enzyme-linked Immunosorbent assay-Competitive
• Image 1: DDAVP ELISA Standard Curve
  
  DDAVP ELISA Kit standard curve