1,5-Anhydroglucitol ELISA Kit (OKCD02247)

• Product Number: OKCD02247
• Name: 1,5-Anhydroglucitol ELISA Kit (OKCD02247)
• Alias Symbols: 1 1,5-Anhydro-D-glucitol; 1,5-Anhydroglucitol; 154-58-5; 1,5-ANHYDROSORBITOL
• Description of Target: 1, 5-Anhydrosorbitol or 1, 5-anhydroglucitol (1, 5-AG) is a validated marker of short-term glycemic control. This substance is derived mainly from food, is well absorbed in the intestine, and is distributed to all organs and tissues. It is metabolically stable, being excreted in the urine when its level exceeds the renal threshold. It is reabsorbed in the renal tubules and is competitively inhibited by glucosuria, which leads to a reduction in its level in serum. The correlation between this reduction and the amount of glucose present in urine is so close that 1, 5 AG can be used as a sensitive, day-to-day, real-time marker of glycemic control. It provides useful information on current glycemic control and is superior to both hemoglobin A1C and fructosamine in detecting near-normoglycemia. 1, 5-AG in human plasma has been proposed for several years as a short-term, retrospective marker of glycemic control and seems to be the most suitable parameter for monitoring glucose excursions. The decrease in serum 1, 5-AG is very sensitive to urinary glucose excretion. It is a metabolically inert polyol that competes with glucose for reabsorption in the kidneys. Otherwise stable levels of 1, 5-AG are rapidly depleted as blood glucose levels exceed the renal threshold for glucosuria. 1, 5-AG is also more tightly associated with glucose fluctuations and postprandial glucose.
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Additional Information: PubChem: 64960
• Assay Info: Assay Methodology: Competitive Inhibition Immunoassay
• Sensitivity: < 0.7 ug/mL
• Protein Name: 1,5-Anhydroglucitol
• Sample Type: Serum, plasma and other biological fluids.
• ELISA Kit Component:

  Component	Amount
  Anti-1,5-Anhydroglucitol Microplate	96 Wells (12 x 8 Well Strips)
  1,5-Anhydroglucitol Lyophilized Standard	2
  1,5-Anhydroglucitol Biotin Complex	1
  100X Avidin/HRP Conjugate	120 uL
  Standard Diluent	1 x 20 mL
  Biotin Complex Diluent	1 x 12 mL
  Avidin/HRP Conjugate Diluent	1 x 12 mL
  30X Wash Buffer	1 x 20 mL
  TMB Substrate	1 x 9 mL
  Stop Solution	1 x 6 mL
  Biotin Complex Reconstitution Buffer	1 x 300 uL

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level 1,5-Anhydroglucitol (1,5-AG) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level 1,5-Anhydroglucitol (1,5-AG) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Range: 1.9-150ug/mL
• ELISA Kit Duration: 2h
• ELISA Kit Principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to 1,5-Anhydroglucitol (1,5-AG) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled 1,5-Anhydroglucitol (1,5-AG) and unlabeled 1,5-Anhydroglucitol (1,5-AG) (Standards or samples) with the pre-coated antibody specific to 1,5-Anhydroglucitol (1,5-AG). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of 1,5-Anhydroglucitol (1,5-AG) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of 1,5-Anhydroglucitol (1,5-AG) in the sample.
• ELISA Kit Linearity:

  Matrix	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	89-98%	78-97%	79-97%	96-103%
  EDTA plasma(n=5)	88-98%	86-93%	78-101%	93-105%
  heparin plasma(n=5)	99-105%	86-104%	78-94%	91-99%

• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	92-105	97
  EDTA plasma(n=5)	94-101	97
  heparin plasma(n=5)	95-102	99

• ELISA Kit Detection Method: Colorimetric
• COA Datasheet: Click Hereto download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
• Gene Symbol: 1,5-AG
• Bulk Note: Please contact customer service when ordering 10+ kits.
• Predicted Species Reactivity: All Species
• Application: Enzyme-linked Immunosorbent assay-Competitive
• Image 1: 1,5-AG ELISA Standard Curve
  
  1,5-AG ELISA Kit standard curve