ABCA13 ELISA Kit (Mouse) (OKCD01208)

• Product Number: OKCD01208
• Name: ABCA13 ELISA Kit (Mouse) (OKCD01208)
• Protein Size (# AA): 5034 amino acids
• NCBI Gene Id: 268379
• Gene Full Name: ATP-binding cassette, sub-family A (ABC1), member 13
• Alias Symbols: 9830132L24;A930002G16Rik;AI956815;ATP-binding cassette sub-family A member 13;ATP-binding cassette transporter.
• Description of Target: ATP-binding cassette sub-family A member 13
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Specificity: This assay has high sensitivity and excellent specificity for detection of ATP Binding Cassette Transporter A13 (ABCA13). No significant cross-reactivity or interference between ATP Binding Cassette Transporter A13 (ABCA13) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay
• Sensitivity: < 33 pg/mL
• Uniprot ID: Q5SSE9
• Protein Name: ATP-binding cassette sub-family A member 13
• Protein Accession #: NP_839990
• Sample Type: Tissue homogenates, cell lysates and other biological fluids.
• Nucleotide Accession #: NM_178259.3
• ELISA Kit Component:

  Component	Amount
  Anti-Abca13 Microplate	96 Wells (12 x 8 Well Strips)
  Abca13 Lyophilized Standard	2 x 5 ng
  100X Biotinylated Abca13 Detector Antibody	120 uL
  Avidin/HRP Conjugate	120 uL
  Standard Diluent	1 x 20 mL
  Detector Antibody Diluent	1 x 12 mL
  Conjugate Diluent	1 x 12 mL
  30X Wash Buffer	1 x 20 mL
  TMB Substrate	1 x 9 mL
  Stop Solution	1 x 6 mL

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ATP Binding Cassette Transporter A13 (ABCA13) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ATP Binding Cassette Transporter A13 (ABCA13) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Range: 78.12-5000pg/mL
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATP Binding Cassette Transporter A13 (ABCA13). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to ATP Binding Cassette Transporter A13 (ABCA13). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATP Binding Cassette Transporter A13 (ABCA13), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ATP Binding Cassette Transporter A13 (ABCA13) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  	90-101%	83-104%	81-98%	91-101%

• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  	95-102	99

• ELISA Kit Detection Method: Colorimetric
• Gene Symbol: Abca13
• Bulk Note: Please contact customer service when ordering 10+ kits.
• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• Image 1: Abca13 ELISA
  
  Mouse Abca13 ELISA Kit standard curve