ABCA3 ELISA Kit (Human) (OKCD01207)

• Product Number: OKCD01207
• Name: ABCA3 ELISA Kit (Human) (OKCD01207)
• Protein Size (# AA): 1704 amino acids
• NCBI Gene Id: 21
• Gene Full Name: ATP binding cassette subfamily A member 3
• Alias Symbols: ABC transporter 3;ABC3;ABC-C;ABC-C transporter;ATP-binding cassette sub-family A member 3;ATP-binding cassette transporter 3;ATP-binding cassette, sub-family A (ABC1), member 3;EST111653;LBM180;phospholipid-transporting ATPase ABCA3;SMDP3;xenobiotic-transporting ATPase ABCA3.
• Description of Target: Plays an important role in the formation of pulmonary surfactant, probably by transporting lipids such as cholesterol.
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Specificity: This assay has high sensitivity and excellent specificity for detection of ATP Binding Cassette Transporter A3 (ABCA3). No significant cross-reactivity or interference between ATP Binding Cassette Transporter A3 (ABCA3) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay
• Sensitivity: < 33 pg/mL
• Uniprot ID: Q99758
• Protein Name: ATP-binding cassette sub-family A member 3
• Protein Accession #: NP_001080
• Sample Type: Tissue homogenates and other biological fluids.
• Nucleotide Accession #: NM_001089.2
• ELISA Kit Component:

  Component	Amount
  Anti-ABCA3 Microplate	96 Wells (12 x 8 Well Strips)
  ABCA3 Lyophilized Standard	2 x 5 ng
  100X Biotinylated ABCA3 Detector Antibody	120 uL
  Avidin/HRP Conjugate	120 uL
  Standard Diluent	1 x 20 mL
  Detector Antibody Diluent	1 x 12 mL
  Conjugate Diluent	1 x 12 mL
  30X Wash Buffer	1 x 20 mL
  TMB Substrate	1 x 9 mL
  Stop Solution	1 x 6 mL

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ATP Binding Cassette Transporter A3 (ABCA3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ATP Binding Cassette Transporter A3 (ABCA3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATP Binding Cassette Transporter A3 (ABCA3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to ATP Binding Cassette Transporter A3 (ABCA3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATP Binding Cassette Transporter A3 (ABCA3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ATP Binding Cassette Transporter A3 (ABCA3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Detection Method: Colorimetric
• Gene Symbol: ABCA3
• Bulk Note: Please contact customer service when ordering 10+ kits.
• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• Image 1: ABCA3 ELISA
  
  Human ABCA3 ELISA Kit standard curve