ABCA4 ELISA Kit (Human) (OKCD00914)

• Product Number: OKCD00914
• Name: ABCA4 ELISA Kit (Human) (OKCD00914)
• Protein Size (# AA): 2273 amino acids
• NCBI Gene Id: 24
• Gene Full Name: ATP binding cassette subfamily A member 4
• Alias Symbols: ABC10;ABCR;ARMD2;ATP binding cassette transporter;ATP-binding cassette sub-family A member 4;ATP-binding cassette transporter, retinal-specific;ATP-binding cassette, sub-family A (ABC1), member 4;ATP-binding transporter, retina-specific;CORD3;FFM;photoreceptor rim protein;retinal-specific ATP-binding cassette transporter;retinal-specific phospholipid-transporting ATPase ABCA4;retina-specific ABC transporter;RIM ABC transporter;RIM protein;RMP;RP19;stargardt disease protein;STGD;STGD1.
• Description of Target: In the visual cycle, acts as an inward-directed retinoid flipase, retinoid substrates imported by ABCA4 from the extracellular or intradiscal (rod) membrane surfaces to the cytoplasmic membrane surface are all-trans-retinaldehyde (ATR) and N-retinyl-phosphatidyl-ethanolamine (NR-PE). Once transported to the cytoplasmic surface, ATR is reduced to vitamin A by trans-retinol dehydrogenase (tRDH) and then transferred to the retinal pigment epithelium (RPE) where it is converted to 11-cis-retinal. May play a role in photoresponse, removing ATR/NR-PE from the extracellular photoreceptor surfaces during bleach recovery.
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Specificity: This assay has high sensitivity and excellent specificity for detection of ATP Binding Cassette Transporter A4 (ABCA4). No significant cross-reactivity or interference between ATP Binding Cassette Transporter A4 (ABCA4) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay
• Sensitivity: < 0.128 ng/mL
• Uniprot ID: P78363
• Protein Name: Retinal-specific ATP-binding cassette transporter
• Protein Accession #: NP_000341
• Sample Type: Tissue homogenates, cell lysates and other biological fluids.
• Nucleotide Accession #: NM_000350.2
• ELISA Kit Component:

  Component	Amount
  Anti-ABCA4 Microplate	96 Wells (12 x 8 Well Strips)
  ABCA4 Lyophilized Standard	2 x 20 ng
  100X Biotinylated ABCA4 Detector Antibody	120 uL
  Avidin/HRP Conjugate	120 uL
  Standard Diluent	1 x 20 mL
  Detector Antibody Diluent	1 x 12 mL
  Conjugate Diluent	1 x 12 mL
  30X Wash Buffer	1 x 20 mL
  TMB Substrate	1 x 9 mL
  Stop Solution	1 x 6 mL

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ATP Binding Cassette Transporter A4 (ABCA4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ATP Binding Cassette Transporter A4 (ABCA4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATP Binding Cassette Transporter A4 (ABCA4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to ATP Binding Cassette Transporter A4 (ABCA4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATP Binding Cassette Transporter A4 (ABCA4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ATP Binding Cassette Transporter A4 (ABCA4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Detection Method: Colorimetric
• COA Datasheet: ClickHereto download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
• Gene Symbol: ABCA4
• Bulk Note: Please contact customer service when ordering 10+ kits.
• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• Image 1: ABCA4 ELISA
  
  Human ABCA4 ELISA Kit standard curve