LRRC3C ELISA Kit (Human) (OKCD00648)

• Product Number: OKCD00648
• Name: LRRC3C ELISA Kit (Human) (OKCD00648)
• Protein Size (# AA): 275 amino acids
• NCBI Gene Id: 100505591
• Gene Full Name: leucine rich repeat containing 3C
• Alias Symbols: leucine-rich repeat-containing protein 3C;putative LRRC3-like protein ENSP00000367157.
• Description of Target: Leucine-rich repeat-containing protein 3C
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Specificity: This assay has high sensitivity and excellent specificity for detection of Leucine Rich Repeat Containing Protein 3C (LRRC3C). No significant cross-reactivity or interference between Leucine Rich Repeat Containing Protein 3C (LRRC3C) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay
• Sensitivity: < 0.066 ng/mL
• Uniprot ID: A6NJW4
• Protein Name: Leucine-rich repeat-containing protein 3C
• Protein Accession #: NP_001182474
• Sample Type: Tissue homogenates and other biological fluids.
• Nucleotide Accession #: NM_001195545.1
• ELISA Kit Component:

  Component	Amount
  Anti-LRRC3C Microplate	96 Wells (12 x 8 Well Strips)
  LRRC3C Lyophilized Standard	2 x 10 ng
  100X Biotinylated LRRC3C Detector Antibody	120 uL
  Avidin/HRP Conjugate	120 uL
  Standard Diluent	1 x 20 mL
  Detector Antibody Diluent	1 x 12 mL
  Conjugate Diluent	1 x 12 mL
  30X Wash Buffer	1 x 20 mL
  TMB Substrate	1 x 9 mL
  Stop Solution	1 x 6 mL

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Leucine Rich Repeat Containing Protein 3C (LRRC3C) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Leucine Rich Repeat Containing Protein 3C (LRRC3C) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Leucine Rich Repeat Containing Protein 3C (LRRC3C). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Leucine Rich Repeat Containing Protein 3C (LRRC3C). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Leucine Rich Repeat Containing Protein 3C (LRRC3C), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Leucine Rich Repeat Containing Protein 3C (LRRC3C) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Detection Method: Colorimetric
• Gene Symbol: LRRC3C
• Bulk Note: Please contact customer service when ordering 10+ kits.
• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• Image 1: LRRC3C ELISA
  
  Human LRRC3C ELISA Kit standard curve