ZBED9 ELISA Kit (Human) (OKCD00647)

Data Sheet
 
Product Number OKCD00647
Product Page www.avivasysbio.com/zbed9-elisa-kit-human-okcd00647.html
Name ZBED9 ELISA Kit (Human) (OKCD00647)
Protein Size (# AA) 1325 amino acids
NCBI Gene Id 114821
Gene Full Name zinc finger BED-type containing 9
Alias Symbols Buster4;dJ1186N24.3;SCAN domain containing 3;SCAN domain-containing protein 3;SCAND3;transposon-derived Buster4 transposase-like protein;ZFP38-L;zinc finger BED domain-containing protein 9;zinc finger protein 305 pseudogene 2;zinc finger protein 452;ZNF305P2;ZNF452.
Description of Target SCAN domain-containing protein 3
Reconstitution and Storage 2°C to 8°C|-20°C
Datasheets/Manuals Printable datasheet for ZBED9 ELISA Kit (Human) (OKCD00647)
Specificity This assay has high sensitivity and excellent specificity for detection of SCAN Domain Containing Protein 3 (SCAND3). No significant cross-reactivity or interference between SCAN Domain Containing Protein 3 (SCAND3) and analogues was observed.
Assay Info Assay Methodology: Quantitative Sandwich Immunoassay
Sensitivity < 0.059 ng/mL
Uniprot ID Q6R2W3
Protein Name SCAN domain-containing protein 3
Protein Accession # NP_443155
Sample Type Serum, plasma, tissue homogenates and other biological fluids.
Nucleotide Accession # NM_052923.1
ELISA Kit Component
ComponentAmount
Anti-ZBED9 Microplate96 Wells (12 x 8 Well Strips)
ZBED9 Lyophilized Standard2 x 10 ng
100X Biotinylated ZBED9 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
ELISA Kit Reproducibility Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level SCAN Domain Containing Protein 3 (SCAND3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level SCAN Domain Containing Protein 3 (SCAND3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Range 0.156-10ng/mL
ELISA Kit Duration 3h
ELISA Kit Principle The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to SCAN Domain Containing Protein 3 (SCAND3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to SCAN Domain Containing Protein 3 (SCAND3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SCAN Domain Containing Protein 3 (SCAND3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of SCAN Domain Containing Protein 3 (SCAND3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)98-105%80-92%78-96%91-99%
EDTA plasma(n=5)88-98%80-97%91-105%83-94%
heparin plasma(n=5)98-105%97-105%93-105%85-99%
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)99-105102
EDTA plasma(n=5)80-8984
heparin plasma(n=5)87-9792
ELISA Kit Detection Method Colorimetric
Gene Symbol ZBED9
Bulk Note Please contact customer service when ordering 10+ kits.
Predicted Species Reactivity Homo sapiens|Human
Application Enzyme-linked Immunosorbent assay-Sandwich
Image 1
ZBED9 ELISA
Human ZBED9 ELISA Kit standard curve
 

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