KIAA1524 ELISA Kit (Human) (OKCD00646)

• Product Number: OKCD00646
• Name: KIAA1524 ELISA Kit (Human) (OKCD00646)
• Protein Size (# AA): 905 amino acids
• NCBI Gene Id: 57650
• Gene Full Name: cellular inhibitor of PP2A
• Alias Symbols: cancerous inhibitor of PP2A;cancerous inhibitor of protein phosphatase 2A;cell proliferation regulating inhibitor of protein phosphatase 2A;KIAA1524;NOCIVA;p90;p90 autoantigen;protein CIP2A.
• Description of Target: Oncoprotein that inhibits PP2A and stabilizes MYC in human malignancies. Promotes anchorage-independent cell growth and tumor formation.
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Specificity: This assay has high sensitivity and excellent specificity for detection of Cancerous Inhibitor Of PP2A (CIP2A). No significant cross-reactivity or interference between Cancerous Inhibitor Of PP2A (CIP2A) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay
• Sensitivity: < 0.056 ng/mL
• Uniprot ID: Q8TCG1
• Protein Name: Protein CIP2A
• Protein Accession #: NP_065941
• Sample Type: Serum, plasma and other biological fluids
• Nucleotide Accession #: NM_020890.2
• ELISA Kit Component:

  Component	Amount
  Anti-KIAA1524 Microplate	96 Wells (12 x 8 Well Strips)
  KIAA1524 Lyophilized Standard	2 x 10 ng
  100X Biotinylated KIAA1524 Detector Antibody	120 uL
  Avidin/HRP Conjugate	120 uL
  Standard Diluent	1 x 20 mL
  Detector Antibody Diluent	1 x 12 mL
  Conjugate Diluent	1 x 12 mL
  30X Wash Buffer	1 x 20 mL
  TMB Substrate	1 x 9 mL
  Stop Solution	1 x 6 mL

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cancerous Inhibitor Of PP2A (CIP2A) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cancerous Inhibitor Of PP2A (CIP2A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cancerous Inhibitor Of PP2A (CIP2A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cancerous Inhibitor Of PP2A (CIP2A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cancerous Inhibitor Of PP2A (CIP2A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cancerous Inhibitor Of PP2A (CIP2A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	79-98%	84-93%	92-99%	79-99%
  EDTA plasma(n=5)	79-89%	80-98%	93-105%	95-104%
  heparin plasma(n=5)	83-102%	80-94%	80-93%	79-98%

• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	98-105	101
  EDTA plasma(n=5)	92-101	96
  heparin plasma(n=5)	84-92	87

• ELISA Kit Detection Method: Colorimetric
• Gene Symbol: CIP2A
• Bulk Note: Please contact customer service when ordering 10+ kits.
• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• Image 1: KIAA1524 ELISA
  
  Human KIAA1524 ELISA Kit standard curve