Product Number |
OKCD00645 |
Product Page |
www.avivasysbio.com/slfn5-elisa-kit-human-okcd00645.html |
Name |
SLFN5 ELISA Kit (Human) (OKCD00645) |
Protein Size (# AA) |
891 amino acids |
NCBI Gene Id |
162394 |
Gene Full Name |
schlafen family member 5 |
Alias Symbols |
schlafen family member 5. |
Description of Target |
May have a role in hematopoietic cell differentiation. |
Reconstitution and Storage |
2°C to 8°C|-20°C |
Datasheets/Manuals |
Printable datasheet for SLFN5 ELISA Kit (Human) (OKCD00645) |
Specificity |
This assay has high sensitivity and excellent specificity for detection of Schlafen Family Member 5 (SLFN5).
No significant cross-reactivity or interference between Schlafen Family Member 5 (SLFN5) and analogues was observed. |
Assay Info |
Assay Methodology: Quantitative Sandwich Immunoassay |
Sensitivity |
< 0.067 ng/mL |
Uniprot ID |
Q08AF3 |
Protein Name |
Schlafen family member 5 |
Protein Accession # |
NP_659412 |
Sample Type |
Serum, plasma, tissue homogenates and other biological fluids. |
Nucleotide Accession # |
NM_144975.3 |
ELISA Kit Component |
Component | Amount |
Anti-SLFN5 Microplate | 96 Wells (12 x 8 Well Strips) |
SLFN5 Lyophilized Standard | 2 x 10 ng |
100X Biotinylated SLFN5 Detector Antibody | 120 uL |
Avidin/HRP Conjugate | 120 uL |
Standard Diluent | 1 x 20 mL |
Detector Antibody Diluent | 1 x 12 mL |
Conjugate Diluent | 1 x 12 mL |
30X Wash Buffer | 1 x 20 mL |
TMB Substrate | 1 x 9 mL |
Stop Solution | 1 x 6 mL |
|
ELISA Kit Reproducibility |
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Schlafen Family Member 5 (SLFN5) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Schlafen Family Member 5 (SLFN5) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% |
ELISA Kit Range |
0.156-10ng/mL |
ELISA Kit Duration |
3h |
ELISA Kit Principle |
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Schlafen Family Member 5 (SLFN5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Schlafen Family Member 5 (SLFN5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Schlafen Family Member 5 (SLFN5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Schlafen Family Member 5 (SLFN5) in the samples is then determined by comparing the O.D. of the samples to the standard curve. |
ELISA Kit Linearity |
Sample | 1:2 Dilution | 1:4 Dilution | 1:8 Dilution | 1:16 Dilution |
serum(n=5) | 88-96% | 81-92% | 88-95% | 80-96% |
EDTA plasma(n=5) | 89-104% | 78-97% | 82-98% | 81-89% |
heparin plasma(n=5) | 83-98% | 79-90% | 97-104% | 79-94% |
|
ELISA Kit Recovery |
Matrix | Recovery Range (%) | Average (%) |
serum(n=5) | 97-105 | 102 |
EDTA plasma(n=5) | 84-96 | 92 |
heparin plasma(n=5) | 86-101 | 94 |
|
ELISA Kit Detection Method |
Colorimetric |
Gene Symbol |
SLFN5 |
Bulk Note |
Please contact customer service when ordering 10+ kits. |
Predicted Species Reactivity |
Homo sapiens|Human |
Application |
Enzyme-linked Immunosorbent assay-Sandwich |
Image 1 | SLFN5 ELISA
| Human SLFN5 ELISA Kit standard curve |
|