MARC1 ELISA Kit (Human) (OKCD00644)

• Product Number: OKCD00644
• Name: MARC1 ELISA Kit (Human) (OKCD00644)
• Protein Size (# AA): 337 amino acids
• NCBI Gene Id: 64757
• Gene Full Name: mitochondrial amidoxime reducing component 1
• Alias Symbols: MARC1;mitochondrial amidoxime-reducing component 1;moco sulfurase C-terminal domain-containing protein 1;MOCO sulphurase C-terminal domain containing 1;molybdenum cofactor sulfurase C-terminal domain-containing protein 1;MOSC domain-containing protein 1, mitochondrial;MOSC1.
• Description of Target: As a component of an N-hydroxylated prodrug-converting complex required to reduce N-hydroxylated prodrugs, such as benzamidoxime. Also able to reduce N(omega)-hydroxy-L-arginine (NOHA) and N(omega)-hydroxy-N(delta)-methyl-L-arginine (NHAM) into L-arginine and N(delta)-methyl-L-arginine, respectively.
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Specificity: This assay has high sensitivity and excellent specificity for detection of MOCO Sulphurase C-Terminal Domain Containing Protein 1 (MOSC1). No significant cross-reactivity or interference between MOCO Sulphurase C-Terminal Domain Containing Protein 1 (MOSC1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay
• Sensitivity: < 0.064 ng/mL
• Uniprot ID: Q5VT66
• Protein Name: Mitochondrial amidoxime-reducing component 1
• Protein Accession #: NP_073583
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
• Nucleotide Accession #: NM_022746.3
• ELISA Kit Component:

  Component	Amount
  Anti-MARC1 Microplate	96 Wells (12 x 8 Well Strips)
  MARC1 Lyophilized Standard	2 x 10 ng
  100X Biotinylated MARC1 Detector Antibody	120 uL
  Avidin/HRP Conjugate	120 uL
  Standard Diluent	1 x 20 mL
  Detector Antibody Diluent	1 x 12 mL
  Conjugate Diluent	1 x 12 mL
  30X Wash Buffer	1 x 20 mL
  TMB Substrate	1 x 9 mL
  Stop Solution	1 x 6 mL

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level MOCO Sulphurase C-Terminal Domain Containing Protein 1 (MOSC1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level MOCO Sulphurase C-Terminal Domain Containing Protein 1 (MOSC1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to MOCO Sulphurase C-Terminal Domain Containing Protein 1 (MOSC1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to MOCO Sulphurase C-Terminal Domain Containing Protein 1 (MOSC1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MOCO Sulphurase C-Terminal Domain Containing Protein 1 (MOSC1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of MOCO Sulphurase C-Terminal Domain Containing Protein 1 (MOSC1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	82-90%	87-95%	78-91%	79-99%
  EDTA plasma(n=5)	91-99%	92-99%	80-103%	91-98%
  heparin plasma(n=5)	86-94%	79-98%	97-105%	81-98%

• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	96-103	101
  EDTA plasma(n=5)	81-98	93
  heparin plasma(n=5)	86-104	92

• ELISA Kit Detection Method: Colorimetric
• Gene Symbol: MTARC1
• Bulk Note: Please contact customer service when ordering 10+ kits.
• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• Image 1: MARC1 ELISA
  
  Human MARC1 ELISA Kit standard curve