PI16 ELISA Kit (Human) (OKCD00642)

• Product Number: OKCD00642
• Name: PI16 ELISA Kit (Human) (OKCD00642)
• Protein Size (# AA): 463 amino acids
• NCBI Gene Id: 221476
• Gene Full Name: peptidase inhibitor 16
• Alias Symbols: CD364;CRISP9;cysteine-rich secretory protein 9;microseminoprotein, beta-binding protein;MSMBBP;peptidase inhibitor 16;protease inhibitor 16;PSP94-binding protein;PSPBP.
• Description of Target: May inhibit cardiomyocyte growth.
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Specificity: This assay has high sensitivity and excellent specificity for detection of Peptidase Inhibitor 16 (PI16). No significant cross-reactivity or interference between Peptidase Inhibitor 16 (PI16) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay
• Sensitivity: < 0.064 ng/mL
• Uniprot ID: Q6UXB8
• Protein Name: Peptidase inhibitor 16
• Protein Accession #: NP_001186088
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Nucleotide Accession #: NM_001199159.1
• ELISA Kit Component:

  Component	Amount
  Anti-PI16 Microplate	96 Wells (12 x 8 Well Strips)
  PI16 Lyophilized Standard	2 x 10 ng
  100X Biotinylated PI16 Detector Antibody	120 uL
  Avidin/HRP Conjugate	120 uL
  Standard Diluent	1 x 20 mL
  Detector Antibody Diluent	1 x 12 mL
  Conjugate Diluent	1 x 12 mL
  30X Wash Buffer	1 x 20 mL
  TMB Substrate	1 x 9 mL
  Stop Solution	1 x 6 mL

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Peptidase Inhibitor 16 (PI16) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Peptidase Inhibitor 16 (PI16) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Range: 1.56-100ng/mL
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Peptidase Inhibitor 16 (PI16). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Peptidase Inhibitor 16 (PI16). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Peptidase Inhibitor 16 (PI16), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Peptidase Inhibitor 16 (PI16) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	83-97%	83-101%	90-103%	79-103%
  EDTA plasma(n=5)	86-99%	92-101%	91-105%	93-101%
  heparin plasma(n=5)	87-101%	79-92%	78-99%	85-97%

• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	80-93	84
  EDTA plasma(n=5)	98-105	102
  heparin plasma(n=5)	80-104	96

• ELISA Kit Detection Method: Colorimetric
• Gene Symbol: PI16
• Bulk Note: Please contact customer service when ordering 10+ kits.
• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• Image 1: PI16 ELISA
  
  Human PI16 ELISA Kit standard curve