SEMA4B ELISA Kit (Human) (OKCD00640)

Data Sheet
 
Product Number OKCD00640
Product Page www.avivasysbio.com/sema4b-elisa-kit-human-okcd00640.html
Name SEMA4B ELISA Kit (Human) (OKCD00640)
Protein Size (# AA) 461 amino acids
NCBI Gene Id 10509
Gene Full Name semaphorin 4B
Alias Symbols sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4B;sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, 4B;SEMAC;semaphorin-4B;semaphorin-C;SemC.
Description of Target Inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons.
Reconstitution and Storage 2°C to 8°C|-20°C
Datasheets/Manuals Printable datasheet for SEMA4B ELISA Kit (Human) (OKCD00640)
Specificity This assay has high sensitivity and excellent specificity for detection of Semaphorin 4B (SEMA4B). No significant cross-reactivity or interference between Semaphorin 4B (SEMA4B) and analogues was observed.
Assay Info Assay Methodology: Quantitative Sandwich Immunoassay
Sensitivity < 0.071 ng/mL
Uniprot ID Q9NPR2
Protein Name Semaphorin-4B
Protein Accession # NP_077041
Sample Type Tissue homogenates and other biological fluids.
Nucleotide Accession # NM_024127.2
ELISA Kit Component
ComponentAmount
Anti-SEMA4B Microplate96 Wells (12 x 8 Well Strips)
SEMA4B Lyophilized Standard2 x 10 ng
100X Biotinylated SEMA4B Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
ELISA Kit Reproducibility Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Semaphorin 4B (SEMA4B) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Semaphorin 4B (SEMA4B) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Range 0.156-10ng/mL
ELISA Kit Duration 3h
ELISA Kit Principle The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Semaphorin 4B (SEMA4B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Semaphorin 4B (SEMA4B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Semaphorin 4B (SEMA4B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Semaphorin 4B (SEMA4B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Detection Method Colorimetric
Gene Symbol SEMA4B
Bulk Note Please contact customer service when ordering 10+ kits.
Predicted Species Reactivity Homo sapiens|Human
Application Enzyme-linked Immunosorbent assay-Sandwich
Image 1
SEMA4B ELISA
Human SEMA4B ELISA Kit standard curve
 

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