MOK ELISA Kit (Human) (OKCD00530)

Data Sheet
 
Product Number OKCD00530
Product Page www.avivasysbio.com/mok-elisa-kit-human-okcd00530.html
Name MOK ELISA Kit (Human) (OKCD00530)
Protein Size (# AA) 419 amino acids
NCBI Gene Id 5891
Gene Full Name MOK protein kinase
Alias Symbols MAPK/MAK/MRK overlapping kinase;MOK protein kinase;RAGE;RAGE1;RAGE-1;renal cell carcinoma antigen;renal tumor antigen 1;STK30.
Description of Target Able to phosphorylate several exogenous substrates and to undergo autophosphorylation. Negatively regulates cilium length in a cAMP and mTORC1 signaling-dependent manner.
Reconstitution and Storage 2°C to 8°C|-20°C
Datasheets/Manuals Printable datasheet for MOK ELISA Kit (Human) (OKCD00530)
Specificity This assay has high sensitivity and excellent specificity for detection of Renal Tumor Antigen (RAGE). No significant cross-reactivity or interference between Renal Tumor Antigen (RAGE) and analogues was observed.
Assay Info Assay Methodology: Quantitative Sandwich Immunoassay
Sensitivity < 0.062 ng/mL
Uniprot ID Q9UQ07
Protein Name MAPK/MAK/MRK overlapping kinase
Protein Accession # NP_001258940
Sample Type Tissue homogenates, cell lysates and other biological fluids
Nucleotide Accession # NM_001272011.1
ELISA Kit Component
ComponentAmount
Anti-MOK Microplate96 Wells (12 x 8 Well Strips)
MOK Lyophilized Standard2 x 10 ng
100X Biotinylated MOK Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
ELISA Kit Reproducibility Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Renal Tumor Antigen (RAGE) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Renal Tumor Antigen (RAGE) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Range 0.156-10ng/mL
ELISA Kit Duration 3h
ELISA Kit Principle The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Renal Tumor Antigen (RAGE). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Renal Tumor Antigen (RAGE). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Renal Tumor Antigen (RAGE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Renal Tumor Antigen (RAGE) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Detection Method Colorimetric
Gene Symbol MOK
Bulk Note Please contact customer service when ordering 10+ kits.
Predicted Species Reactivity Homo sapiens|Human
Application Enzyme-linked Immunosorbent assay-Sandwich
Image 1
MOK ELISA
Human MOK ELISA Kit standard curve
 

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