Product Number |
OKCD00530 |
Product Page |
www.avivasysbio.com/mok-elisa-kit-human-okcd00530.html |
Name |
MOK ELISA Kit (Human) (OKCD00530) |
Protein Size (# AA) |
419 amino acids |
NCBI Gene Id |
5891 |
Gene Full Name |
MOK protein kinase |
Alias Symbols |
MAPK/MAK/MRK overlapping kinase;MOK protein kinase;RAGE;RAGE1;RAGE-1;renal cell carcinoma antigen;renal tumor antigen 1;STK30. |
Description of Target |
Able to phosphorylate several exogenous substrates and to undergo autophosphorylation. Negatively regulates cilium length in a cAMP and mTORC1 signaling-dependent manner. |
Reconstitution and Storage |
2°C to 8°C|-20°C |
Datasheets/Manuals |
Printable datasheet for MOK ELISA Kit (Human) (OKCD00530) |
Specificity |
This assay has high sensitivity and excellent specificity for detection of Renal Tumor Antigen (RAGE).
No significant cross-reactivity or interference between Renal Tumor Antigen (RAGE) and analogues was observed. |
Assay Info |
Assay Methodology: Quantitative Sandwich Immunoassay |
Sensitivity |
< 0.062 ng/mL |
Uniprot ID |
Q9UQ07 |
Protein Name |
MAPK/MAK/MRK overlapping kinase |
Protein Accession # |
NP_001258940 |
Sample Type |
Tissue homogenates, cell lysates and other biological fluids |
Nucleotide Accession # |
NM_001272011.1 |
ELISA Kit Component |
Component | Amount |
Anti-MOK Microplate | 96 Wells (12 x 8 Well Strips) |
MOK Lyophilized Standard | 2 x 10 ng |
100X Biotinylated MOK Detector Antibody | 120 uL |
Avidin/HRP Conjugate | 120 uL |
Standard Diluent | 1 x 20 mL |
Detector Antibody Diluent | 1 x 12 mL |
Conjugate Diluent | 1 x 12 mL |
30X Wash Buffer | 1 x 20 mL |
TMB Substrate | 1 x 9 mL |
Stop Solution | 1 x 6 mL |
|
ELISA Kit Reproducibility |
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Renal Tumor Antigen (RAGE) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Renal Tumor Antigen (RAGE) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% |
ELISA Kit Range |
0.156-10ng/mL |
ELISA Kit Duration |
3h |
ELISA Kit Principle |
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Renal Tumor Antigen (RAGE). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Renal Tumor Antigen (RAGE). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Renal Tumor Antigen (RAGE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Renal Tumor Antigen (RAGE) in the samples is then determined by comparing the O.D. of the samples to the standard curve. |
ELISA Kit Detection Method |
Colorimetric |
Gene Symbol |
MOK |
Bulk Note |
Please contact customer service when ordering 10+ kits. |
Predicted Species Reactivity |
Homo sapiens|Human |
Application |
Enzyme-linked Immunosorbent assay-Sandwich |
Image 1 | MOK ELISA
| Human MOK ELISA Kit standard curve |
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