AASDHPPT ELISA Kit (Human) (OKCD00323)

• Product Number: OKCD00323
• Name: AASDHPPT ELISA Kit (Human) (OKCD00323)
• Protein Size (# AA): 309 amino acids
• NCBI Gene Id: 60496
• Gene Full Name: aminoadipate-semialdehyde dehydrogenase-phosphopantetheinyl transferase
• Alias Symbols: 4'-phosphopantetheinyl transferase;AASD-PPT;ACPS;alpha-aminoadipic semialdehyde dehydrogenase-phosphopantetheinyl transferase;CGI-80;holo ACP synthase;holo-[acyl-carrier-protein] synthase;L-aminoadipate-semialdehyde dehydrogenase-phosphopantetheinyl transferase;LYS2;LYS5;LYS5 ortholog.
• Description of Target: Catalyzes the post-translational modification of target proteins by phosphopantetheine. Can transfer the 4'-phosphopantetheine moiety from coenzyme A to a serine residue of a broad range of acceptors, such as the acyl carrier domain of FASN.
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Specificity: This assay has high sensitivity and excellent specificity for detection of Aminoadipate Semialdehyde Phosphopantetheinyl Transferase (AASDHPPT). No significant cross-reactivity or interference between Aminoadipate Semialdehyde Phosphopantetheinyl Transferase (AASDHPPT) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay
• Sensitivity: < 0.054 ng/mL
• Uniprot ID: Q9NRN7
• Protein Name: L-aminoadipate-semialdehyde dehydrogenase-phosphopantetheinyl transferase
• Protein Accession #: NP_056238
• Sample Type: Tissue homogenates and other biological fluids.
• Nucleotide Accession #: NM_015423.2
• ELISA Kit Component:

  Component	Amount
  Anti-AASDHPPT Microplate	96 Wells (12 x 8 Well Strips)
  AASDHPPT Lyophilized Standard	2 x 10 ng
  100X Biotinylated AASDHPPT Detector Antibody	120 uL
  Avidin/HRP Conjugate	120 uL
  Standard Diluent	1 x 20 mL
  Detector Antibody Diluent	1 x 12 mL
  Conjugate Diluent	1 x 12 mL
  30X Wash Buffer	1 x 20 mL
  TMB Substrate	1 x 9 mL
  Stop Solution	1 x 6 mL

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Aminoadipate Semialdehyde Phosphopantetheinyl Transferase (AASDHPPT) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Aminoadipate Semialdehyde Phosphopantetheinyl Transferase (AASDHPPT) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aminoadipate Semialdehyde Phosphopantetheinyl Transferase (AASDHPPT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Aminoadipate Semialdehyde Phosphopantetheinyl Transferase (AASDHPPT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Aminoadipate Semialdehyde Phosphopantetheinyl Transferase (AASDHPPT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Aminoadipate Semialdehyde Phosphopantetheinyl Transferase (AASDHPPT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Detection Method: Colorimetric
• Gene Symbol: AASDHPPT
• Bulk Note: Please contact customer service when ordering 10+ kits.
• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• Image 1: AASDHPPT ELISA
  
  Human AASDHPPT ELISA Kit standard curve