PPP3CA ELISA Kit (Human) (OKCD00274)

• Product Number: OKCD00274
• Name: PPP3CA ELISA Kit (Human) (OKCD00274)
• Protein Size (# AA): 521 amino acids
• NCBI Gene Id: 5530
• Gene Full Name: protein phosphatase 3 catalytic subunit alpha
• Alias Symbols: ACCIID;calcineurin A alpha;Calmodulin-dependent calcineurin A subunit alpha isoform;CALN;CALNA;CALNA1;CAM-PRP catalytic subunit;CCN1;CNA1;DEE91;IECEE;IECEE1;PPP2B;protein phosphatase 3 catalytic subunit alpha isozyme;serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform.
• Description of Target: Calcium-dependent, calmodulin-stimulated protein phosphatase which plays an essential role in the transduction of intracellular Ca(2+)-mediated signals (PubMed:15671020, PubMed:18838687, PubMed:19154138, PubMed:23468591). Many of the substrates contain a PxIxIT motif and/or a LxVP motif (PubMed:17498738, PubMed:17502104, PubMed:23468591, PubMed:27974827, PubMed:22343722). In response to increased Ca(2+) levels, dephosphorylates and activates phosphatase SSH1 which results in cofilin dephosphorylation (PubMed:15671020). In response to increased Ca(2+) levels following mitochondrial depolarization, dephosphorylates DNM1L inducing DNM1L translocation to the mitochondrion (PubMed:18838687). Dephosphorylates heat shock protein HSPB1 (By similarity). Dephosphorylates and activates transcription factor NFATC1 (PubMed:19154138). In response to increased Ca(2+) levels, regulates NFAT-mediated transcription probably by dephosphorylating NFAT and promoting its nuclear translocation (PubMed:26248042). Dephosphorylates and inactivates transcription factor ELK1 (PubMed:19154138). Dephosphorylates DARPP32 (PubMed:19154138).
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Specificity: This assay has high sensitivity and excellent specificity for detection of Calcineurin (CaN). No significant cross-reactivity or interference between Calcineurin (CaN) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay
• Sensitivity: < 0.057 ng/mL
• Uniprot ID: Q08209
• Protein Name: Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform
• Protein Accession #: NP_000935
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Nucleotide Accession #: NM_000944.4
• ELISA Kit Component:

  Component	Amount
  Anti-PPP3CA Microplate	96 Wells (12 x 8 Well Strips)
  PPP3CA Lyophilized Standard	2 x 10 ng
  100X Biotinylated PPP3CA Detector Antibody	120 uL
  Avidin/HRP Conjugate	120 uL
  Standard Diluent	1 x 20 mL
  Detector Antibody Diluent	1 x 12 mL
  Conjugate Diluent	1 x 12 mL
  30X Wash Buffer	1 x 20 mL
  TMB Substrate	1 x 9 mL
  Stop Solution	1 x 6 mL

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Calcineurin (CaN) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Calcineurin (CaN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calcineurin (CaN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Calcineurin (CaN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Calcineurin (CaN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Calcineurin (CaN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Detection Method: Colorimetric
• Gene Symbol: PPP3CA
• Bulk Note: Please contact customer service when ordering 10+ kits.
• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• Image 1: PPP3CA ELISA
  
  Human PPP3CA ELISA Kit standard curve