| Product Number |
OKBB00380 |
| Product Page |
www.avivasysbio.com/il-29-elisa-kit-human-okbb00380.html |
| Name |
IL-29 ELISA Kit (Human) (OKBB00380) |
| NCBI Gene Id |
282618 |
| Gene Full Name |
interferon, lambda 1 |
| Description |
For quantitative detection of human IL-29 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
| Alias Symbols |
Cytokine ZCYTO21, IFN lambda 1, IFN-lambda-1, IFNL1, IL 29, IL-29, IL29, IL29_HUMAN, Interferon lambda 1, Interferon lambda-1, Interferon, lambda 1, Interleukin 29, Interleukin-29 |
| Description of Target |
Interferon lambda-1, also called Interleukin-29 (IL-29) is a protein that in humans is encoded by the IL29 gene. IL-29 is a member of the helical cytokine family and is a type III interferon. This gene is mapped to 19q13.2. It is a cytokine with immunomodulatory activity. This gene may play a role in antiviral immunity. It can up-regulates MHC class I antigen expression. It is the ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IFNLR1. The ligand/receptor complex seems to signal through the Jak-STAT pathway. IL-29 plays an important role in host defenses against microbes and its gene is highly upregulated in cells infected with viruses. |
| Reconstitution and Storage |
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.) |
| Datasheets/Manuals |
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
| Specificity |
Natural and recombinant human IL-29 |
| Immunogen |
Expression system for standard: NS0; Immunogen sequence: G20-T200 |
| Assay Info |
Assay Methodology: Quantitative Sandwich Immunoassay |
| Sensitivity |
<10 pg/ml |
| Uniprot ID |
Q8IU54 |
| Protein Name |
Interferon lambda-1 |
| Protein Accession # |
NP_742152.1 |
| Sample Type |
Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) |
| Nucleotide Accession # |
NM_172140.1 |
| ELISA Kit Component |
| Component | Amount |
|---|
| 96-Well plate Pre-coated with Anti-Human IL-29 Antibody | 1 x (12 x 8 Well Strip) | | Lyophilized Recombinant Human IL-29 Standard | 2 x 10 ng/tube | | 100X Biotinylated Anti-Human IL-29 Antibody | 1 x 130 uL | | 100X Avidin-Biotin-Peroxidase Complex (ABC) | 1 x 130 uL | | Sample Diluent Buffer | 1 x 30 mL | | Antibody Diluent Buffer | 1 x 12 mL | | ABC Diluent Buffer | 1 x 12 mL | | TMB Color Developing Agent | 1 x 10 mL | | TMB Stop Solution | 1 x 10 mL | | 10X Wash Buffer | 1 x 30 mL |
|
| ELISA Kit Reproducibility |
| Intra-Assay Precision | Inter-Assay Precision |
|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 | | n | 16 | 16 | 16 | 24 | 24 | 24 | | Mean (pg/mL) | 517 | 1446 | 2336 | 643 | 1550 | 2468 | | SD | 33.1 | 76.64 | 114.5 | 45.01 | 105.4 | 140.7 | | CV% | 6.4 | 5.3 | 4.9 | 7 | 6.8 | 5.7 |
|
| ELISA Kit Range |
62.5 pg/ml - 4,000 pg/ml |
| ELISA Kit Duration |
2.5 Hours |
| ELISA Kit Principle |
Aviva's human IL-29 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-29 has been precoated onto 96-well plates. Standards(NS0, G20-T200) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-29 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human IL-29 amount of sample captured in plate. |
| ELISA Kit Detection Method |
Colorimetric, OD450 nm |
| Gene Symbol |
IFNL1 |
| Predicted Species Reactivity |
Human |
| Application |
ELISA |
| Image 1 | IL-29 ELISA
 | Human IL-29 ELISA Kit standard curve |
|
