MBNL1 Protein Lysate (OCOA20348)

Data Sheet
 
Product Number OCOA20348
Product Page www.avivasysbio.com/mbnl1-protein-lysate-ocoa20348.html
Name MBNL1 Protein Lysate (OCOA20348)
Molecular Weight 41.6 kDa
Tag C-MYC/DDK
NCBI Gene Id 4154
Host HEK293T
Alias Symbols EXP, MBNL
Product Format Lyophilized
Reconstitution and Storage The lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
Datasheets/Manuals Printable datasheet for OCOA20348
Additional Information Components: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
Additional Information Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Protein Accession # NP_997176
Nucleotide Accession # NM_207293
Gene Symbol MBNL1
Predicted Species Reactivity Human
 

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