ACOT12 Protein Lysate (OCOA06713)

Data Sheet
 
Product Number OCOA06713
Product Page www.avivasysbio.com/acot12-protein-lysate-ocoa06713.html
Name ACOT12 Protein Lysate (OCOA06713)
Molecular Weight 61.9 kDa
Tag C-MYC/DDK
NCBI Gene Id 134526
Host HEK293T
Gene Full Name acyl-CoA thioesterase 12
Description ACOT12 HEK293T cell transient overexpression lysate (as WB positive control)
Alias Symbols acetyl-coenzyme A thioesterase;acyl-CoA thioester hydrolase 12;acyl-coenzyme A thioesterase 12;Cach;CACH-1;cytoplasmic acetyl-CoA hydrolase 1;cytosolic acetyl-CoA hydrolase;hCACH-1;STARD15;StAR-related lipid transfer (START) domain containing 15;START domain-containing protein 15;THEAL.
Product Format Lyophilized
Description of Target Acyl-coenzyme A thioesterase 12, acyl-coenzyme A thioesterase 12 [Homo sapiens], Homo sapiens acyl-CoA thioesterase 12 (ACOT12), mRNA
Reconstitution and Storage The lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
Datasheets/Manuals Printable datasheet for ACOT12 Protein Lysate (OCOA06713)
Additional Information Components: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
Additional Information Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Uniprot ID https://www.uniprot.org/uniprot/Q8WYK0
Protein Name NM_130767, NP_570123, Q8WYK0
Protein Accession # https://www.ncbi.nlm.nih.gov/protein/NP_570123
Nucleotide Accession # https://www.ncbi.nlm.nih.gov/nuccore/NM_130767
Gene Symbol ACOT12
Predicted Species Reactivity Homo sapiens
 

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