ME1 Protein Lysate (OCOA00770)

Data Sheet
 
Product Number OCOA00770
Product Page www.avivasysbio.com/me1-protein-lysate-ocoa00770.html
Name ME1 Protein Lysate (OCOA00770)
Molecular Weight 64.1 kDa
Tag C-MYC/DDK
NCBI Gene Id 4199
Host HEK293T
Gene Full Name malic enzyme 1
Description ME1 HEK293T cell transient overexpression lysate (as WB positive control)
Alias Symbols HUMNDME;malate dehydrogenase (oxaloacetate-decarboxylating) (NADP(+));Malic enzyme 1;malic enzyme 1, NADP(+)-dependent, cytosolic;malic enzyme 1, soluble;Malic enzyme, cytoplasmic;MES;NADP-dependent malic enzyme;NADP-ME;pyruvic-malic carboxylase.
Product Format Lyophilized
Description of Target Homo sapiens malic enzyme 1 (ME1), mRNA, NADP-dependent malic enzyme, NADP-dependent malic enzyme [Homo sapiens]
Reconstitution and Storage The lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
Datasheets/Manuals Printable datasheet for ME1 Protein Lysate (OCOA00770)
Additional Information Components: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
Additional Information Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Uniprot ID https://www.uniprot.org/uniprot/P48163
Protein Name NM_002395, NP_002386, P48163
Protein Accession # https://www.ncbi.nlm.nih.gov/protein/NP_002386
Nucleotide Accession # https://www.ncbi.nlm.nih.gov/nuccore/NM_002395
Gene Symbol ME1
Predicted Species Reactivity Homo sapiens
 

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