| Product Number |
OCEG01209 |
| Product Page |
www.avivasysbio.com/human-brain-tissue-cell-lysate-oceg01209.html |
| Name |
Human Brain Tissue Cell Lysate (OCEG01209) |
| Concentration |
2 mg/ml |
| Product Format |
Liquid 0.063 M Tris-HCl (pH 6.8), 2% SDS, 50mM DTT, 0.002% bromophenol blue with 10% glycerol |
| Reconstitution and Storage |
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles |
| Datasheets/Manuals |
Printable datasheet for OCEG01209 |
| Application Info |
Human brain tissue lysate is ready to load on SDS-PAGE for Western blotting. |
| Preparation |
Brain tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 g/ml of aprotinin, 5 g/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
| Purification |
Antigen Immunoaffiinity Purification |
| Predicted Species Reactivity |
Human |
| Application |
WB |
|
