CD44/CD44 Antibody (OATA00643)

Data Sheet
 
Product Number OATA00643
Product Page www.avivasysbio.com/cd44-cd44-antibody-oata00643.html
Name CD44/CD44 Antibody (OATA00643)
Clone IM7
Isotype Rat IgG2b, kappa
NCBI Gene Id 12505; 960
Host Rat
Clonality Monoclonal
Gene Full Name CD44 molecule (Indian blood group)
Alias Symbols Pgp, HERM, Ly-2, Ly-24, Pgp-1, HERMES, AU023126, AW121933, AW146109
Product Format Liquid. 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Reference

Chandler HL, Haeussler DJ, Gemensky-Metzler AJ, Wilkie DA, and Lutz EA. 2012. Invest. Ophthalmol. Vis. Sci. 53:1835-1845. (in vitro blocking, canine)

Lee L-F, Logronio K, Tu GH, Zhai W, Ni I, Mei L, Dilley J, Yu J, et al. 2012. Proc. Natl. Acad. Sci. 10.1073. (Flow cytometry).

Ruffell B, Poon GFT, Lee SSM, Brown KL, Tjew S-L, Cooper J, and Johnson P. 2011. J. Biol. Chem. 286:19179-19190. (Immunoprecipitation)

Miyake Y, Matsumoto H, Yokoo M, Miyazawa K et al. 2006. Biol. Reprod. 74: 501-510. (Immunohistochemistry - frozen tissue, swine)

Veir JK, Lappin MR, and Dow SW. 2006. Journal of Feline Medicine and Surgery. 8:400-411. (Flow cytometry - feline)

Frank NY, Margaryan A, Huang Y, Schatton T, Waaga-Gasser AM, Gasser M, Sayegh MH, Sadee W, and Frank MH. 2005. Cancer Res. 65:4320-4333. (Immunohistochemistry - frozen tissue)

Fischer A, Schumacher N, Maier M, Sendtner M, and Gessler M. 2004. Genes & Dev. 18:901-911. (Immunohistochemistry - paraffin embedded tissue)

Xu H, Manivannan A, Liversidge J, Sharp PF, Forrester JV, and Crane IJ. 2002. J. Leukoc. Biol. 72:1133-1141. (in vivo functional assays, induction of apoptosis)

Si-Tahar M, Sitaraman S, Shibahara T, and Madara JL. 2001. Am. J. Physiol. Cell Physiol. 280:C423-C432. (in vitro functional assays, Western Blot

Description of Target The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis.
Reconstitution and Storage 2-8C protected from light
Datasheets/Manuals Printable datasheet for OATA00643
Specificity CD44
Additional Information Excitation Laser: Red (633-640 nm)
Application Info This antibody preparation has been quality-tested for flow cytometry using mouse spleen cells, or an appropriate cell type (where indicated). Please refer to the figure legend for the optimal concentration used to stain the tissue shown. We recommend titrating the antibody under your specific conditions to determine the optimal concentration of antibody needed in your experimental system.
Additional Information Emmision: 710 nm
Uniprot ID P16070, P15379
Protein Name CD44 antigen
Protein Accession # NP_000601.3?
Nucleotide Accession # NM_000610.3
Tested Species Reactivity Human, Mouse
Gene Symbol CD44, CD44
Predicted Species Reactivity Chimpanzee, Baboon, Cynomolgus, Rhesus, Swine, Feline, Canine, Equine
Application FC
Image 1
C57Bl/6 splenocytes
C57Bl/6 splenocytes were stained with 0.5 ug redFluor 710 Anti-Hu/Mo CD44 (OATA00643) (solid line) or 0.5 ug redFluor 100 Rat IgG2b isotype control (dashed line).
 

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