ROCK1 Antibody - middle region (OASG06458)

• Product Number: OASG06458
• Name: ROCK1 Antibody - middle region (OASG06458)
• Molecular Weight: 160 kDa
• Isotype: IgG
• NCBI Gene Id: 6093; 19877; 81762
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 1 mg/ml
• Gene Full Name: Rho-associated, coiled-coil containing protein kinase 1
• Alias Symbols: ROCK-I, P160ROCK
• Product Format: Liquid. 1 mg/mL in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• Description of Target: This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. A pseudogene, related to this gene, is also located on chromosome 18.
• Reconstitution and Storage: Store at -20C. Avoid repeated freeze/thaw cycles.
• Specificity: Rock-1 Polyclonal Antibody detects endogenous levels of Rock-1 protein.
• Application Info: WB 1:500-1:2000; IHC 1:100-1:300; Immunocytochemistry 1:200-1:1000; ELISA 1:20000;
• Immunogen: Synthesized peptide derived from the Internal region of human Rock-1.
• Uniprot ID: Q13464, P70335, Q63644
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Gene Symbol: ROCK1
• Predicted Species Reactivity: Human, Mouse, Rat, Monkey
• Application: WB, IHC
• Image 1: COS7
  
  Western Blot using COS7