MTOR Antibody (OASG04742)

• Product Number: OASG04742
• Name: MTOR Antibody (OASG04742)
• Isotype: IgG
• NCBI Gene Id: 2475
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 1 mg/ml
• Gene Full Name: mechanistic target of rapamycin (serine/threonine kinase)
• Alias Symbols: SKS, FRAP, FRAP1, FRAP2, RAFT1, RAPT1
• Product Format: Liquid. PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• Description of Target: Serine/threonine protein kinase which is a central regulator of cellular metabolism, growth and survival in response to hormones, growth factors, nutrients, energy and stress signals. MTOR directly or indirectly regulates the phosphorylation of at least 800 proteins. Functions as part of 2 structurally and functionally distinct signaling complexes mTORC1 and mTORC2 (mTOR complex 1 and 2). Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. This includes phosphorylation of EIF4EBP1 and release of its inhibition toward the elongation initiation factor 4E (eiF4E). Moreover, phosphorylates and activates RPS6KB1 and RPS6KB2 that promote protein synthesis by modulating the activity of their downstream targets including ribosomal protein S6, eukaryotic translation initiation factor EIF4B, and the inhibitor of translation initiation PDCD4. Stimulates the pyrimidine biosynthesis pathway, both by acute regulation through RPS6KB1-mediated phosphorylation of the biosynthetic enzyme CAD, and delayed regulation, through transcriptional enhancement of the pentose phosphate pathway which produces 5-phosphoribosyl-1-pyrophosphate (PRPP), an allosteric activator of CAD at a later step in synthesis, this function is dependent on the mTORC1 complex. Regulates ribosome synthesis by activating RNA polymerase III-dependent transcription through phosphorylation and inhibition of MAF1 an RNA polymerase III-repressor. In parallel to protein synthesis, also regulates lipid synthesis through SREBF1/SREBP1 and LPIN1. To maintain energy homeostasis mTORC1 may also regulate mitochondrial biogenesis through regulation of PPARGC1A. mTORC1 also negatively regulates autophagy through phosphorylation of ULK1.
• Reconstitution and Storage: Store at -20°C. Avoid repeated freeze/thaw cycles.
• Specificity: mTOR Polyclonal Antibody detects endogenous levels of mTOR protein.
• Additional Information: Cellular Localization: Endoplasmic reticulum membrane Golgi apparatus membrane Mitochondrion outer membrane Lysosome Cytoplasm Nucleus, PML body Microsome membrane. Shuttles between cytoplasm and nucleus. Accumulates in the nucleus in response to hypoxia . Targeting to lysosomes depends on amino acid availability and RRAGA and RRAGB .
  Tissue Specificity: Expressed in numerous tissues, with highest levels in testis.
• Application Info: WB: 1:500-1:2000
  IHC: 1:100-1:300
  IF: 1:200-1:1000
  ELISA: 1:40000
  Optimal dilutions should be determined by the end user.
• Immunogen: Synthesized peptide derived between 2390-2470 amino acids from human mTOR around the non-phosphorylation site of S2448.
• Uniprot ID: P42345, Q9JLN9, P42346
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Target Post-Translational Modification: Autophosphorylates when part of mTORC1 or mTORC2. Phosphorylation at Ser-1261, Ser-2159 and Thr-2164 promotes autophosphorylation. Phosphorylation in the kinase domain modulates the interactions of MTOR with RPTOR and PRAS40 and leads to increased intrinsic mTORC1 kinase activity. Phosphorylation at Thr-2173 in the ATP-binding region by AKT1 strongly reduces kinase activity.
• Gene Symbol: MTOR
• Predicted Species Reactivity: Human, Mouse, Rat
• Application: WB, IHC, IF
• Image 1: HUVEC
  
  Western Blot using HUVEC
• Image 2: Western Blot
  
  Western Blot