| Product Number |
OASG00647 |
| Product Page |
www.avivasysbio.com/acly-antibody-phospho-ser455-oasg00647.html |
| Name |
ACLY Antibody (Phospho-Ser455) (OASG00647) |
| Molecular Weight |
125 kDa |
| Isotype |
IgG |
| NCBI Gene Id |
47 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
1 mg/ml |
| Gene Full Name |
ATP citrate lyase |
| Description |
|
| Alias Symbols |
ACL, ATPCL, CLATP |
| Product Format |
Liquid PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Description of Target |
ATP-citrate synthase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. Has a central role in de novo lipid synthesis. In nervous tissue it may be involved in the biosynthesis of acetylcholine. |
| Reconstitution and Storage |
Store at -20°C. Avoid repeated freeze/thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for anti-ACLY (OASG00647) antibody |
| Specificity |
Phospho-ATP-citrate synthase (S455) Polyclonal Antibody detects endogenous levels of ATP-citrate synthase protein only when phosphorylated at S455. |
| Additional Information |
Cellular Localization: Cytoplasm. |
| Application Info |
WB: 1:500~2000
IHC: 1:100~300
ELISA: 1:10000
Optimal dilutions should be determined by the end user. |
| Immunogen |
Synthesized peptide derived between 390-470 aa from human ATP-citrate synthase around the phosphorylation site of S455. |
| Uniprot ID |
P53396, Q91V92, P16638 |
| Publications |
Modulation of matrix metabolism by ATP-citrate lyase in articular chondrocytes. J Biol Chem. 293, 12259-12270 (2018). 29929979 |
| Purification |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Target Post-Translational Modification |
ISGylated. Acetylated at Lys-540, Lys-546 and Lys-554 by KAT2B/PCAF. Acetylation is promoted by glucose and stabilizes the protein, probably by preventing ubiquitination at the same sites. Acetylation promotes de novo lipid synthesis. Deacetylated by SIRT2. Ubiquitinated at Lys-540, Lys-546 and Lys-554 by UBR4, leading to its degradation. Ubiquitination is probably inhibited by acetylation at same site (Probable). |
| Gene Symbol |
ACLY |
| Predicted Species Reactivity |
Human, Mouse, Rat, Monkey |
| Application |
WB, IHC, ELISA |
| Image 1 | |
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