TCP1 alpha-alpha Antibody (OASE00325)

• Product Number: OASE00325
• Name: TCP1 alpha-alpha Antibody (OASE00325)
• Clone: 91a
• Isotype: IgG2a
• Conjugation: Unconjugated
• NCBI Gene Id: 21454
• Host: Rat
• Clonality: Monoclonal
• Concentration: 1 mg/ml
• Gene Full Name: t-complex protein 1
• Description: T-complex polypeptide-1 (TCP1) is a ~60 kDa protein constitutively expressed in almost all eukaryotic cells, and is up-regulated during spermatogenesis. It is found in the cytosol as a subunit of a hetero-oligomeric chaperone that is known to be involved in the folding of actin and tubulin. The family of proteins termed chaperonins act to recognize and stabilize polypeptide intermediates during folding, assembly and disassembly, and share many characteristics with Heat Shock Protein 70 (HSP 70) including high abundance, induction by environmental stress, and ATPase activity. The chaperonin family includes the mitochondrial HSP60, Escherichia coli GroEL, the plastid Rubisco-subunit binding protein, and the archaebacterial protein TF55. The TCP1 sequence shows nearly 40% identity to TF55, but only minimal similarity to HSP60 and GroEL.
• Alias Symbols: p63, Tcp-1, TCP1 alpha, Ccta, TRic, Tp63, 21454, c-cpn, CCT, Tcp1, ccpn, AI528772, Cct1, Tcp 1, c cpn
• Reconstitution and Storage: Store at -20C. Shipping with Blue Ice or 4C.
• Specificity: Detects ~60 & ~92kDa. Cross reactivity with human HSP60 in WB. Reacts weakly with S. cerevisiae, consistent with the epitope being AKLRS (instead of AKLRA). In C. elegans, it reacts with TCP1 alpha and another CCT subunit. In plants, it recog TCP1 of P. sativum, and the sequence of A. thalania TCP1 over the region of the epitope AKLRA. It has also been shown that it reacts with a subunit of a specialized chaperonin which folds phytochrome.
• Additional Information: Background Info: Detects a 60kDa protein corresponding to the molecular weight of TCP1 alpha; also detects a molecular mass band of ~92kDa. Cross reactivity with human Hsp60 has been observed with this antibody in immunoblot analysis. Reacts weakly with Saccharomyces cerevisiae, consistent with the epitope sequence being AKLRS (instead of AKLRA). In C. elegans, it reacts with TCP1 alpha and another CCT subunit. In plants, it recognizes TCP1 of Pisum sativum, and the sequence of Arabidopsis thalania TCP1 over the region of the epitope AKLRA. It has also been shown that it reacts with a subunit of a specialized chaperonin which folds phytochrome.
• Additional Information: Scientific Background: T-complex polypeptide-1 (TCP1) is a ~60 kDa protein constitutively expressed in almost all eukaryotic cells, and is up-regulated during spermatogenesis. It is found in the cytosol as a subunit of a hetero-oligomeric chaperone that is known to be involved in the folding of actin and tubulin. The family of proteins termed chaperonins act to recognize and stabilize polypeptide intermediates during folding, assembly and disassembly, and share many characteristics with Heat Shock Protein 70 (HSP 70) including high abundance, induction by environmental stress, and ATPase activity. The chaperonin family includes the mitochondrial HSP60,Escherichia coli GroEL, the plastid Rubisco-subunit binding protein, and the archaebacterial protein TF55. The TCP1 sequence shows nearly 40% identity to TF55, but only minimal similarity to HSP60 and GroEL.
  
• Immunogen: Recombinant Mouse TCP1 alpha protein fragment (carboxy terminal region).
• Additional Information: Certificate of Analysis: 1ug/mL was sufficient for detection of TCP1 alpha in 20 ug of 3T3 cell lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody.
• Dilution: WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
• Storage Buffer: PBS pH7.4, 50% glycerol, 0.1% sodium azide
• Uniprot ID: P11983
• Protein Accession #: NP_038714.2
• Purification: Protein G Purified
• Gene Symbol: TCP1 alpha
• Predicted Species Reactivity: Human, Mouse, Rat, Bovine, Rabbit, Guinea Pig, Hamster, Dog, Pig, Yeast, C. elegans, Drosophila, Monkey
• Application: WB, ICC, IF, IP, FC
• Image 1: Immunofluorescence
  
  Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Hsp40 Polyclonal Antibody. Tissue: Heat Shocked Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Hsp40 Polyclonal Antibody at 1:100 for 12 hours at 4C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Hsp40 Antibody. (C) Composite. Heat Shocked at 42C for 1h.
• Image 2: Immunofluorescence
  
  Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-GRP94 Polyclonal Antibody. Tissue: Heat Shocked Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-GRP94 Polyclonal Antibody at 1:120 for 12 hours at 4C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Melanosome. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-GRP94 Antibody. (C) Composite. Heat Shocked at 42C for 1h.
• Image 3: Western Blot
  
  Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of HSP40 protein using Rabbit Anti-HSP40 Polyclonal Antibody. Primary Antibody: Rabbit Anti-HSP40 Polyclonal Antibody at 1:1000.