VDAC1 Antibody (OASE00304)

• Product Number: OASE00304
• Name: VDAC1 Antibody (OASE00304)
• Clone: S152B-23
• Isotype: IgG2a
• Conjugation: Unconjugated
• NCBI Gene Id: 7416
• Host: Mouse
• Clonality: Monoclonal
• Concentration: 1 mg/ml
• Gene Full Name: voltage-dependent anion channel 1
• Description: Voltage-dependent anion-selective channel protein 1 (also known as VDAC, VDAC1 or outer mitochondrial membrane protein porin 1) is the the outer mitochondrial membrane receptor for hexokinase and BCL2L1. VDAC forms a channel through the mitochondrial membrane and is involved in small molecule diffusion, cell volume regulation and apoptosis. VDAC may participate in the formation of the permeability transition pore complex (PTPC), which is responsible for the release of mitochondrial products that triggers apoptosis.
• Alias Symbols: Voltage Dependent Anion Channel 1, Porin, Voltage dependent anion selective channel protein 1, Voltage-dependent anion-selective channel protein 1, hVDAC1, MGC111064, Mitochondrial Porin, Outer mitochondrial membrane protein porin 1, Plasmalemmal porin, Porin 31HL, Porin 31HM, PORIN-31-HL, VDAC 1, VDAC, VDAC-1
• Reconstitution and Storage: Store at -20C. Shipping with Blue Ice or 4C.
• Specificity: Detects ~30kDa. Does not cross-react with VDAC2 or VDAC3 (based on KO validation results).
• Additional Information: Background Info: Detects a 30 kDa protein. Does not cross-react with VDAC2 or VDAC3 (based on KO validation results)
• Additional Information: Scientific Background: Voltage-dependent anion-selective channel protein 1 (also known as VDAC, VDAC1 or outer mitochondrial membrane protein porin 1) is the the outer mitochondrial membrane receptor for hexokinase and BCL2L1. VDAC forms a channel through the mitochondrial membrane and is involved in small molecule diffusion, cell volume regulation and apoptosis. VDAC may participate in the formation of the permeability transition pore complex (PTPC), which is responsible for the release of mitochondrial products that triggers apoptosis.
• Immunogen: Fusion protein amino acids 1-283 (full-length) of human VDAC1. Mouse: 98% identity (279/283 amino acids identical). Rat: 98% identity (279/283 amino acids identical) >60% identity with VDAC2 and VDAC3.
• Additional Information: Certificate of Analysis: 1ug/mL was sufficient for detection of VDAC1 in 20 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Dilution: WB (1:1000); optimal dilutions for assays should be determined by the user.
• Storage Buffer: PBS pH 7.4, 50% glycerol, 0.1% sodium azide
• Uniprot ID: P21796
• Protein Accession #: NP_003365.1.
• Purification: Protein G Purified
• Gene Symbol: VDAC1
• Predicted Species Reactivity: Human, Mouse, Rat
• Application: WB, IHC, ICC, IF
• Image 1: Immunofluorescence
  
  Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SHANK1/SHANK3 Monoclonal Antibody, Clone S367-51. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-SHANK1/SHANK3 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) SHANK1/SHANK3 Antibody. (D) Composite.
• Image 2: Western Blot
  
  Western Blot analysis of Monkey COS cells transfected with HA-tagged Shank1 showing detection of ~190 kDa SHANK1/SHANK3 protein using Mouse Anti-SHANK1/SHANK3 Monoclonal Antibody, Clone S367-51. Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with HA-tagged Shank1. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-SHANK1/SHANK3 Monoclonal Antibody at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~190 kDa.