Product Number |
OASE00288 |
Product Page |
www.avivasysbio.com/glua1-glur1-glutamate-receptor-antibody-oase00288.html |
Name |
GluA1/GluR1 Glutamate Receptor Antibody (OASE00288) |
Clone |
S355-1 |
Isotype |
IgG1 |
Conjugation |
Unconjugated |
NCBI Gene Id |
50592 |
Host |
Mouse |
Clonality |
Monoclonal |
Concentration |
1 mg/ml |
Gene Full Name |
glutamate receptor, ionotropic, AMPA 1 |
Description |
Glutamic acid is the major excitatory neurotransmitter in the mammalian central nervous system. Glutamate receptors are classified on the basis of their activation by different agonists (1-3). GluR1, human glutamate receptor type 1, is an integral membrane protein that is widely expressed in the human brain. The postsynaptic actions of glutamic acid are mediated by a variety of receptors that are named according to their selective agonists. GluR1 is known to bind a kainate subtype of agonist. It has been found that malfunctioning of the glutamatergic system may result in certain brain disorders and neurodegeneration (3). |
Alias Symbols |
AMPA 1, AMPA selective glutamate receptor 1, Glutamate receptor 1, GluA1, GLUH 1, GluR K1, GluRK1, Glr-1, Glur-1, HIPA, Glur1, GluR-A, AI853806, 2900051M01Rik, GluRA, Glr1, MGC13325, GLUH1, GluA1, GLUR1, HBGR1, GLURA, gluR-, GluA1, Glutamate receptor ionotropic AMPA 1, Glutamate receptor ionotropic, Gria 1, HBGR1, OTTHUMP00000160643, OTTHUMP00000165781, OTTHUMP00000224241, OTTHUMP00000224242, OTTHUMP00000224243 |
Reference |
1. Potier M.C., Spillantini M.G., Carter N.P. (1992) DNA Seq. 2(4): 211-218. 2. Puckett, C., et al. (1991) Proc. Nat. Acad. Sci. USA 88(17): 7557-7561. 3. Gregor, P., et al. (1993) Proc. Nat. Acad. Sci. USA 90: 3053-3057.
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Reconstitution and Storage |
Store at -20C. Shipping with Blue Ice or 4C. |
Datasheets/Manuals |
Printable datasheet for OASE00288 |
Specificity |
Detects ~100kDa. Does not cross-react with GluR2. |
Additional Information |
Background Info: Detects ~100kDa. Does not cross-react with GluR2. |
Additional Information |
Scientific Background: Glutamic acid is the major excitatory neurotransmitter in the mammalian central nervous system. Glutamate receptors are classified on the basis of their activation by different agonists (1-3). GluR1, human glutamate receptor type 1, is an integral membrane protein that is widely expressed in the human brain. The postsynaptic actions of glutamic acid are mediated by a variety of receptors that are named according to their selective agonists. GluR1 is known to bind a kainate subtype of agonist. It has been found that malfunctioning of the glutamatergic system may result in certain brain disorders and neurodegeneration (3). |
Immunogen |
Fusion protein amino acids 1-389 (extracellular N-terminus) of rat GluA1/GluR1 |
Additional Information |
Certificate of Analysis: 1ug/mL was sufficient for detection of GluA1/GluR1 in 20ug of mouse brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody. |
Dilution |
WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user. |
Storage Buffer |
PBS pH 7.4, 50% glycerol, 0.1% sodium azide |
Uniprot ID |
P19490 |
Protein Accession # |
NP_113796 |
Purification |
Protein G Purified |
Gene Symbol |
GluR1 |
Predicted Species Reactivity |
Human, Mouse, Rat |
Application |
WB, IHC, ICC, IF |
Image 1 | Immunofluorescence
| Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GFAP R416WT Monoclonal Antibody, Clone S206B-9. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GFAP R416WT Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) GFAP R416WT Antibody. (D) Composite. |
| Image 2 | Western Blot
| Western Blot analysis of Rat Brain Membrane showing detection of GFAP protein using Mouse Anti-GFAP Monoclonal Antibody, Clone S206B-9. Primary Antibody: Mouse Anti-GFAP Monoclonal Antibody at 1:250. |
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