GluA1/GluR1 Glutamate Receptor Antibody (OASE00288)

Data Sheet
 
Product Number OASE00288
Product Page www.avivasysbio.com/glua1-glur1-glutamate-receptor-antibody-oase00288.html
Name GluA1/GluR1 Glutamate Receptor Antibody (OASE00288)
Clone S355-1
Isotype IgG1
Conjugation Unconjugated
NCBI Gene Id 50592
Host Mouse
Clonality Monoclonal
Concentration 1 mg/ml
Gene Full Name glutamate receptor, ionotropic, AMPA 1
Description Glutamic acid is the major excitatory neurotransmitter in the mammalian central nervous system. Glutamate receptors are classified on the basis of their activation by different agonists (1-3). GluR1, human glutamate receptor type 1, is an integral membrane protein that is widely expressed in the human brain. The postsynaptic actions of glutamic acid are mediated by a variety of receptors that are named according to their selective agonists. GluR1 is known to bind a kainate subtype of agonist. It has been found that malfunctioning of the glutamatergic system may result in certain brain disorders and neurodegeneration (3).
Alias Symbols AMPA 1, AMPA selective glutamate receptor 1, Glutamate receptor 1, GluA1, GLUH 1, GluR K1, GluRK1, Glr-1, Glur-1, HIPA, Glur1, GluR-A, AI853806, 2900051M01Rik, GluRA, Glr1, MGC13325, GLUH1, GluA1, GLUR1, HBGR1, GLURA, gluR-, GluA1, Glutamate receptor ionotropic AMPA 1, Glutamate receptor ionotropic, Gria 1, HBGR1, OTTHUMP00000160643, OTTHUMP00000165781, OTTHUMP00000224241, OTTHUMP00000224242, OTTHUMP00000224243
Reference 1. Potier M.C., Spillantini M.G., Carter N.P. (1992) DNA Seq. 2(4): 211-218.
2. Puckett, C., et al. (1991) Proc. Nat. Acad. Sci. USA 88(17): 7557-7561.
3. Gregor, P., et al. (1993) Proc. Nat. Acad. Sci. USA 90: 3053-3057.
Reconstitution and Storage Store at -20C. Shipping with Blue Ice or 4C.
Datasheets/Manuals Printable datasheet for OASE00288
Specificity Detects ~100kDa. Does not cross-react with GluR2.
Additional Information Background Info: Detects ~100kDa. Does not cross-react with GluR2.
Additional Information Scientific Background: Glutamic acid is the major excitatory neurotransmitter in the mammalian central nervous system. Glutamate receptors are classified on the basis of their activation by different agonists (1-3). GluR1, human glutamate receptor type 1, is an integral membrane protein that is widely expressed in the human brain. The postsynaptic actions of glutamic acid are mediated by a variety of receptors that are named according to their selective agonists. GluR1 is known to bind a kainate subtype of agonist. It has been found that malfunctioning of the glutamatergic system may result in certain brain disorders and neurodegeneration (3).
Immunogen Fusion protein amino acids 1-389 (extracellular N-terminus) of rat GluA1/GluR1
Additional Information Certificate of Analysis: 1ug/mL was sufficient for detection of GluA1/GluR1 in 20ug of mouse brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Dilution WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Storage Buffer PBS pH 7.4, 50% glycerol, 0.1% sodium azide
Uniprot ID P19490
Protein Accession # NP_113796
Purification Protein G Purified
Gene Symbol GluR1
Predicted Species Reactivity Human, Mouse, Rat
Application WB, IHC, ICC, IF
Image 1
Immunofluorescence
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GFAP R416WT Monoclonal Antibody, Clone S206B-9. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GFAP R416WT Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) GFAP R416WT Antibody. (D) Composite.
Image 2
Western Blot
Western Blot analysis of Rat Brain Membrane showing detection of GFAP protein using Mouse Anti-GFAP Monoclonal Antibody, Clone S206B-9. Primary Antibody: Mouse Anti-GFAP Monoclonal Antibody at 1:250.
 

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