SUR1 Antibody (OASE00257)

• Product Number: OASE00257
• Name: SUR1 Antibody (OASE00257)
• Clone: S289-16
• Isotype: IgG1
• Conjugation: Unconjugated
• NCBI Gene Id: 25559
• Host: Mouse
• Clonality: Monoclonal
• Concentration: 1 mg/ml
• Gene Full Name: ATP-binding cassette, subfamily C (CFTR/MRP), member 8
• Description: Sulfonylurea receptors (SUR) are membrane proteins which are the molecular targets of the sulfonylurea class of anti-diabetic drugs whose mechanism of action is to promote insulin release from pancreatic beta cells. More specifically, SUR proteins are subunits of the inward-rectifier potassium ion channels Kir6.x (6.1 and 6.2) (1). The association of four Kir6.x and four SUR subunits form an ion conducting channel commonly referred to as the KATP channel. The primary function of the sulfonylurea receptor is to sense intracellular levels of the nucleotides ATP and ADP and in response facilitate the open or closing its associated Kir6.x potassium channel. Hence the KATP channel monitors the energy balance within the cell (2).
• Alias Symbols: AM60008PU-N, ABC36, Abcc8, ATP binding cassette sub family C member 8, HHF1, HRINS, MRP8, PHHI, SUR, SUR1, Sulfonylurea receptor (hyperinsulinemia), ATP binding cassette sub family C (CFTR/MRP) member 8, ATP binding cassette transporter sub family C member 8 (1), ATP-binding cassette sub-family C member 8, HI, PHHI, Sulfonylurea receptor 1, SUR1delta2, TNDM2
• Reference: 1.     Campbell J.D., Sansom M.S., Ashcroft F.M. (2003) EMBO Resp. 4(11): 1038-1042. 2.     Nichols C.G. (2006) Nature. 440 (7083): 470-476.
• Reconstitution and Storage: Store at -20C. Shipping with Blue Ice or 4C.
• Specificity: Detects ~160kDa. Does not cross-react with SUR2B.
• Additional Information: Background Info: Detects ~160kDa. Does not cross-react with SUR2B
• Additional Information: Scientific Background: Sulfonylurea receptors (SUR) are membrane proteins which are the molecular targets of the sulfonylurea class of anti-diabetic drugs whose mechanism of action is to promote insulin release from pancreatic beta cells. More specifically, SUR proteins are subunits of the inward-rectifier potassium ion channels Kir6.x (6.1 and 6.2) (1). The association of four Kir6.x and four SUR subunits form an ion conducting channel commonly referred to as the KATP channel. The primary function of the sulfonylurea receptor is to sense intracellular levels of the nucleotides ATP and ADP and in response facilitate the open or closing its associated Kir6.x potassium channel. Hence the KATP channel monitors the energy balance within the cell (2).
• Immunogen: Fusion protein amino acids 1548-1582 (cytoplasmic C-terminus) of rat SUR1
• Additional Information: Certificate of Analysis: 1ug/mL was sufficient for detection of SUR1 in 20ug of mouse brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
• Dilution: WB (1:1000), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
• Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide
• Uniprot ID: Q09429
• Protein Accession #: NP_037171.2
• Purification: Protein G Purified
• Gene Symbol: SUR1
• Predicted Species Reactivity: Human, Mouse, Rat, Hamster
• Application: WB, IHC, ICC, IF
• Image 1: Immunofluorescence
  
  Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GIT1 Monoclonal Antibody, Clone S39B-8. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GIT1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) GIT1 Antibody. (D) Composite.
• Image 2: Western Blot
  
  Western Blot analysis of Rat brain membrane lysate showing detection of GIT1 protein using Mouse Anti-GIT1 Monoclonal Antibody, Clone S39B-8. Primary Antibody: Mouse Anti-GIT1 Monoclonal Antibody at 1:1000.