GABA-B Receptor 1 Antibody (OASE00251)

Data Sheet
 
Product Number OASE00251
Product Page www.avivasysbio.com/gaba-b-receptor-1-antibody-oase00251.html
Name GABA-B Receptor 1 Antibody (OASE00251)
Clone S93A-49
Isotype IgG1
Conjugation Unconjugated
NCBI Gene Id 81657
Host Mouse
Clonality Monoclonal
Concentration 1 mg/ml
Gene Full Name gamma-aminobutyric acid (GABA) B receptor 1
Description GABA (γ-aminobutyric acid) is the primary inhibitory neurotransmitter in the central nervous system and interacts with three different receptors: GABA(A), GABA(B) and GABA(C) receptor. The ionotropic GABA(A) and GABA(C) receptors are ligand-gated ion channels that produce fast inhibitory synaptic transmission. In contrast, the metabotropic GABA(B) receptor is coupled to G proteins that modulate slow inhibitory synaptic transmission (1). Functional GABA(B) receptors form heterodimers of GABA(B)R1 and GABA(B)R2 where GABA(B)R1 binds the ligand and GABA(B)R2 is the primary G protein contact site (2). Two isoforms of GABA(B)R1 have been cloned: GABA(B)R1a is a 130 kD protein and GABA(B)R1b is a 95 kD protein (3). G proteins subsequently inhibit adenyl cylase activity and modulate inositol phospholipid hydrolysis. GABA(B) receptors have both pre- and postsynaptic inhibitions: presynaptic GABA(B) receptors inhibit neurotransmitter release through suppression of high threshold calcium channels, while postsynaptic GABA(B) receptors inhibit through coupled activation of inwardly rectifying potassium channels. In addition to synaptic inhibition, GABA(B) receptors may also be involved in hippocampal long-term potentiation, slow wave sleep and muscle relaxation (1).
Alias Symbols GABA-B receptor 1, GABA-B-R1, GABR1_Human, Gamma aminobutyric acid receptor 1, GB1, GPRC3A
Reference 1. Jones K.A. et al. (2000) Neuropsychopharmacology 23: 2. Duthey B. et al. (2002) J Biol Chem. 277: 3236-41.
3. Kaupmann K. et al. (1997) Nature 386: 239-46.
Reconstitution and Storage Store at -20C. Shipping with Blue Ice or 4C.
Datasheets/Manuals Printable datasheet for OASE00251
Specificity Detects ~115kDa. No cross-reactivity against GABA(B)R2.
Additional Information Background Info: Detects ~115kDa. No cross-reactivity against GABA(B)R2
Additional Information Scientific Background: GABA (?-aminobutyric acid) is the primary inhibitory neurotransmitter in the central nervous system and interacts with three different receptors: GABA(A), GABA(B) and GABA(C) receptor. The ionotropic GABA(A) and GABA(C) receptors are ligand-gated ion channels that produce fast inhibitory synaptic transmission. In contrast, the metabotropic GABA(B) receptor is coupled to G proteins that modulate slow inhibitory synaptic transmission (1). Functional GABA(B) receptors form heterodimers of GABA(B)R1 and GABA(B)R2 where GABA(B)R1 binds the ligand and GABA(B)R2 is the primary G protein contact site (2). Two isoforms of GABA(B)R1 have been cloned: GABA(B)R1a is a 130 kD protein and GABA(B)R1b is a 95 kD protein (3). G proteins subsequently inhibit adenyl cylase activity and modulate inositol phospholipid hydrolysis. GABA(B) receptors have both pre- and postsynaptic inhibitions: presynaptic GABA(B) receptors inhibit neurotransmitter release through suppression of high threshold calcium channels, while postsynaptic GABA(B) receptors inhibit through coupled activation of inwardly rectifying potassium channels. In addition to synaptic inhibition, GABA(B) receptors may also be involved in hippocampal long-term potentiation, slow wave sleep and muscle relaxation (1).
Immunogen Fusion protein amino acids 873-977 (cytoplasmic C-terminus) of rat GABA(B)R1
Additional Information Certificate of Analysis: 1ug/mL was sufficient for detection of GABA(B)R1 in 20ug of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Dilution WB (1:1000); optimal dilutions for assays should be determined by the user.
Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide
Uniprot ID Q9Z0U4
Protein Accession # NP_112290.2
Purification Protein G Purified
Gene Symbol GABA B Receptor 1
Predicted Species Reactivity Human, Mouse, Rat
Application WB, ICC, IF
Image 1
Immunofluorescence
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Cav3.1 Monoclonal Antibody, Clone S178A-9. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Cav3.1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Membrane, Cytoplasm, Nucleoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Cav3.1 Antibody. (D) Composite.
Image 2
Western Blot
Western Blot analysis of Rat brain membrane lysate showing detection of Cav3.1 Calcium Channel protein using Mouse Anti-Cav3.1 Calcium Channel Monoclonal Antibody, Clone S178A-9. Primary Antibody: Mouse Anti-Cav3.1 Calcium Channel Monoclonal Antibody at 1:1000.
 

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