Hsp47 Antibody (OASE00148)

Data Sheet
 
Product Number OASE00148
Product Page www.avivasysbio.com/hsp47-antibody-oase00148.html
Name Hsp47 Antibody (OASE00148)
Clone 1C4-1A6
Isotype IgG1 Kappa
Conjugation Unconjugated
NCBI Gene Id 871
Host Mouse
Clonality Monoclonal
Concentration 1 mg/ml
Gene Full Name serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1)
Description HSP47 is a chaperone protein, member of the superfamily of serine proteinase inhibitors. Also known as SERPINH1, a serine proteinase inhibitor. It is a stress protein that resides in the endoplasmic reticulum, has an active role on the intracellular process of folding, assembly and secretion of pro-collagens. Recent studies have shown the association of on an increased expression of HSP47 around fibrotic lesions (1). The identification of a novel biomarker on cell therapies aimed to reduce the progression of fibrotic diseases, could be used potentially as a universal marker, since HSP47 binds a single substrate (2). Type I collagen is fundamental during the healing process after a myocardial infarction. It is critical in the position of collagen-produced cells and the assembly of collagen fibrils (3).
Alias Symbols SerpinH1, Colligin, Gp46, serine proteinase inhibitor, cysteine proteinase inhibitor, collagen binding protein
Product Format Liquid PBS (pH 7.4) with 50% glycerol and 0.09% sodium azide
Reference 1. Razzaque MS., Taquchi T., (1999) Histol Histopathol. 14 (4): 1999-212.
2. Taguchi T., et al. (2011) Acta Histochem Cytochem. 44(2):35-41.
3. Nong Z., et al. (2011) Am J Pathol. 197(5): 2189-96.
Description of Target HSP47 is a chaperone protein, member of the superfamily of serine proteinase inhibitors. Also known as SERPINH1, a serine proteinase inhibitor. It is a stress protein that resides in the endoplasmic reticulum, has an active role on the intracellular process of folding, assembly and secretion of pro-collagens. Recent studies have shown the association of on an increased expression of HSP47 around fibrotic lesions. The identification of a novel biomarker on cell therapies aimed to reduce the progression of fibrotic diseases, could be used potentially as a universal marker, since HSP47 binds a single substrate. Type I collagen is fundamental during the healing process after a myocardial infarction. It is critical in the position of collagen-produced cells and the assembly of collagen fibrils.
Reconstitution and Storage Store at -20C. Shipping with Blue Ice or 4C.
Datasheets/Manuals Printable datasheet for OASE00148
Specificity Detects 47kDa.
Additional Information Cellular Localization: Endoplasmic reticulum lumen
Certificate of Analysis: 1 ug/ml of SMC-203 was sufficient for detection of HSP47 in 20 ug of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Application Info WB: 1:1000
ICC/IF: 1:100
Immunogen Human HSP47, full length
Dilution WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide
Uniprot ID P50454.2
Protein Accession # NP_001193943
Purification Protein G Purified
Gene Symbol HSP47
Predicted Species Reactivity Human
Application WB, ICC, IF
Image 1
Immunofluorescence
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Cav1.2 Monoclonal Antibody, Clone S57. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Cav1.2 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Membrane, Cytoplasm, Nucleoplasm. Magnification: 60X. (A) Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain. (B) Anti-Cav1.2 Antibody. (C) Composite. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Cav1.2 Antibody. (D) Composite.
Image 2
Immunohistochemistry
Immunohistochemistry analysis using Mouse Anti-CaV1.2 Calcium Channel Monoclonal Antibody, Clone S57. Tissue: hippocampus. Species: Human. Fixation: 10% formalin. Primary Antibody: Mouse Anti-CaV1.2 Calcium Channel Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.
Image 3
Immunohistochemistry
Immunohistochemistry analysis using Mouse Anti-CaV1.2 Calcium channel Monoclonal Antibody, Clone S57-47. Tissue: Brain Tissue. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-CaV1.2 Calcium channel Monoclonal Antibody at 1:10000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 ul for 2 minutes at RT. Magnification: 40x.
 

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